and 8. The collected cells were diluted to 1 × 106cells/mL with PBS. A cell disruption system (ATPIO, Nanjing, China, ATPIO-1000D) was used to lyse the cells. The lysed cells were assessed at 450 nm to measure the HAT concentration using a chicken HAT ELISA kit (YL, Shangha...
Cytokines levels in supernatants of chSPDCs and co-incubated DC-T-cell in response to rEaGAPDH, LPS, pET-32a protein, or PBS were quantified by ELISA kits. The production of cytokines A IL-12, B IFN‐γ, C IL-10 and D TGF-β in the supernatant of rEaGAPDH-pulsed chSPDCs. The cyto...
The tyrosinase content was measured using an ELISA kit from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The experiment included a blank well, a standard well, and a sample well, and each group included three replicates. Only the chromogenic agent and stop solution were added to ...