CONSTITUTION:In immunological measurement using chemiluminescence reaction of luminol with peroxidase of horseradish and hydrogen peroxide, a phenolic compound represented by 4-(cyanomethylthio)phenol is used as an enhancer for chemiluminescence. A luminous pattern emitted by the above-mentioned constitution ...
;SOLUTION: In analytical and diagnostic chemical methods for detecting various analytes in a biological fluid by detecting and measuring a luminescence generated by a reaction of luminol or substituted luminol with a perborate in an aqueous solvent using a suitable catalyst, a chemiluminescence analysis...
Advanced laboratory techniques for diagnosing Toxoplasma gondii encephalitis in AIDS patients: significance of intrathecal production and comparison with P... The polymerase chain reaction (PCR) for detection of cerebral spinal fluid (CSF) Toxoplasma gondii DNA was combined with the study of intrathecal ...
the concentration of a specific sample according to the luminescence of the chemical reaction.3The generation of CL inside CLIA process is driven by the use of substrates like luminol, isoluminol and alkaline phosphatase(ALP), being the last one of the most commonly used for protein labelling.3...
The present disclosure further provides a method for enhancing luminescence of 1,2-dioxetane compounds, which includes adding the chemiluminescent enhancing reagents of the present disclosure into a solution containing 1,2-dioxetane compounds, or using the kit of the present disclosure. ...
a CL system was designed to detect H2O2on the basis ofB. subtilisspores catalyzed under the luminol-H2O2reaction because of the existence of CotA-laccase on their surface (Scheme 1). A cascade reaction based on B5R18 coupled to the spores was developed for the detection of Glyp with high ...
J. Gold nanoparticle-catalyzed luminol chemiluminescence and its analytical applications. Anal. Chem. 2005, 77, 3324–3329.Search in Google Scholar Zhang, Y.; Li, Y.; Yan, X. P. Aqueous layer-by-layer epitaxy of type-II CdTe/CdSe quantum dots with near-infrared fluorescence for bioimaging ...
Multi-analyte immunoassays on microarrays and on multiplex DNA microarrays have been described for quantitative analysis of small organic molecules (e.g., antibiotics, drugs of abuse, small molecule toxins), proteins (e.g., antibodies or protein toxins), and microorganisms, viruses, and eukaryotic ...
Chemiluminescent substrates (Luminol, H2O2 and enhancer) were purchased from Monobind Inc. (California, USA). HRP Substrate TMB Solutions for ELISA was purchased from Thermo Fisher Scientific Inc. (Rockford, USA). The magnetic particles (2-μm, 0.1%, w/v) modified with amino groups (MP-Amine...
the overall photon emission observed with the guanosine/hemin CL hydrogel was at least one order of magnitude higher with respect to that of the ssDNA designed DNAzyme, when tested with the same amounts of luminol and H2O2, thus suggesting a higher efficiency for the luminol/H2O2 CL reaction....