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每小時天氣-Madison, WI 截至01:59 CST 為止 12月25日星期三 02:00 -7° 3% 陰 體感-7° 風東北東 0 km/h 可見度12 公里 紫外線指數0 (最大值 11) 雲量100% 雪量0 公分 03:00 -7° 3% 陰 體感-7° 風東北東 0 km/h 可見度9 公里 紫外線指數0 (最大值 11) 雲量100% 雪量0...
A dose of glucose (n = 6; 1 g, Dextrose 50%, Hospira, Lake Forest, IL, USA), insulin (n = 6; 20 U, Vetsulin, Merck Animal Health, Madison, NJ, USA), 2-deoxy-D-glucose (n = 6; 60 mg, D8375-1G, Sigma-Aldrich, St. Louis, MO, USA), or saline (n =...
A SNS sodium channel PCR fragment of interdomain region I between positions 1,736 and 1,797 of the SNS sodium channel nucleic acid sequence was subcloned into pGem3Z (Promega. Madison, Wis., USA) and digoxygenin (DIG)-UTP (Boehringer-Mannheim, Germany) labeled sense or antisense cRNA gene...
Human embryonic kidney (HEK) 293 cells were transiently transfected with cDNA coding for GluN1 and GluN2A subunits or GluN1c and GluN2Ac subunits22 and green fluorescent protein (GFP) in the ratio of 1:2:0.5 using ViafectTM reagent (Promega Corporation, Madison, WI) as previously described...
Makielski, Jonathan C. (Fitchburg, WI, US) Ye, Bin (Madison, WI, US) Ackerman, Michael J. (Rochester, MN, US) Application Number: 10/632342 Publication Date: 07/01/2004 Filing Date: 08/01/2003 Export Citation: Click for automatic bibliography generation ...
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Following a 1-min incubation at room temperature, chemiluminescence was quantified with a GloMax-Multi+ detection system (Promega, Madison, WI). Results are presented in relative light units. Surface expression in FRT cells FRT cells were maintained in DMEM/F-12 with 8% fetal bovine serum (...
Samples were incubated with RNase A (Promega, Madison, WI, USA; final concentration ¼ 0.5 mg/ml) and propidium iodide (Sigma-Aldrich; (50 mg/ml) for 2 h at 4 1C before analysis with a flow cytometer and FlowJo software (Tree Star Inc.; Ashland, OR, USA). AnnexinV assay. Cells...
In Proceedings of the Joint Pattern Recognition Symposium, Madison, WI, USA, 17 June 2003; pp. 236–243. [Google Scholar] Le, H.M.; Do, T.-T.; Hoang, T.; Cheung, N.-M. Sdrsac: Semidefinite-based randomized approach for robust point cloud registration without correspondences. In ...