For example, signals belong to two polarizations in polarization multiplexed 20-Gb/s channels which were separated with a PBS in front of the receivers with the help of a polarization controller [29]. This approach is quite simple from a component point of view; however, the real challenge ...
Incubation with PBS instead of primary antibodies served as the negative controls. Finally, the sections were incubated with secondary antibodies at room temperature for 45 minutes. The reaction products were devel- oped by 3, 3′-diaminobenzidine solution with hydrogen peroxide and counter...
2. Domains are the words of the protein universe3, and several dictionaries have been compiled (e.g., SMART, Pfam, SCOP2, CATH). The omics era provided key insight into how multidomain proteins evolve. Rearrangement of existing protein domains4,5,6, mostly at termini...
Following a 90 min centrifugation at 100,000g at 4 °C, NSC-EVs were harvested and resuspended in 100 μL of PBS (Gibco) before being stored at −80 °C. The concentration of NSC-EVs was detected by a BCA protein assay. Dynamic light scattering and TEM were performed to detect the...
Cells were washed with 1x PBS to remove the free extracellular SBFI-AM or PBFI-AM following treatment with TBP2 at different concentrations (0, 1, 2, 5, 8, 20 µM) with 2 h incubation period at 37 °C. The fluorescence of SBFI-AM (λex = 350 nm, λem = 527 nm) or PBFI-...
This opens up an opportunity for us to approach local or state PBS stations about preparing related documentaries about suffrage history. The Minnesota Historical Society is preparing a major exhibit on the American suffrage movement for 2020. The exhibit will first be housed in St. Paul MN at ...
157 Thai PBS feeds F FTA 0 0 tnvchannel2 7-Sep-23 0 013 C 4008 H 15000 4/5 Thaicom 6 (78.5° E) SEAsia QPSK DVB-S2 (8956/40), EIRP Channel T Encryption Package Res. Compression V_PID A_PID SID Owner Date * 158 Thai PBS 3 TV BISS HD MPEG...
The degradation study in vitro showed that 7-channel conduit could hold its apparent geometry for about 12 weeks in phosphate buffer solution (PBS) at 37degreesC, and the pH values of the degradation solution were detected in the range 4.1-4.5. The influences of the conduit architecture on ...
Cells were under a continuous flow of sterile and RNA/DNAse free PBS in order to reduce contamination. After a cell was picked, the glass capillary was broken into a tube containing 10 μl of lysis buffer and DNAse (TaqMan Gene ®Expression Cells-to-CT Kit). For every ...
Afterward, the cells were rinsed twice with ice-cold PBS prior to being visualized using spinning-disc confocal microscopy. The fluorescence was excited by a 488 nm laser and detected through a 525/50 nm filter (Semrock). For flow cytometry analysis, cells were incubated with 2 μM ...