KcsA. KcsA was expressed in freshly made C41 DE3 competent cells (Lucigen Madison, WI) in LB media Luria-Bertani (LB) broth medium, supplemented with 0.5% glycerol (as a chemical chaperone) 0.2% glucose, b0.r4ie mf,gK/mcslAamwapsiceixlltirna,c0t.e1d mwMith(ItPhTe G1.)5a%...