Challenges and limitationsO. Fröbert
Despite its promising future, the field of liquid biopsies is young and still strongly biased by technical and biological limitations. In this review, we will identify the main challenges associated with the use of cell-free long RNAs for the discovery of diagnostic biomarkers. For that purpose,...
limitations of the quantification approach. Based on these findings we designed an advanced barcode construct (BC32) to improved barcode calling and quantification, and to ensure a sensitive detection of even highly diluted barcodes. Our results emphasize the importance of using curated barcode ...
diagnosis of cancer and other diseases. The application of liquid biopsy provides a new possible solution, as it has several advantages such as non-invasive, real-time dynamic monitoring, and overcoming tumor heterogeneity. Liquid biopsy has shown predictive value in immunotherapy, significantly improvin...
from using a sc/snRNA-seq reference dataset from source A to deconvolve a RNA-seq sample from source B and showed these errors can lead to inaccurate estimates of cell composition P for source B, where sources could be distinct donors or studies. Considering the potential limitations of using...
Short interfering RNA therapeutics: nanocarriers, prospects and limitations IET Nanobiotechnol, 9 (2015), pp. 386-395 CrossrefView in ScopusGoogle Scholar 81 T.-H. Tran, G. Mattheolabakis, H. Aldawsari, M. Amiji Exosomes as nanocarriers for immunotherapy of cancer and inflammatory diseases Cli...
discuss the challenges and limitations that need to be overcome, such as off-target effects, safety, and delivery to the tumor site. Finally, we will provide an overview of the current challenges and opportunities in the field of CRISPR-based cancer therapy and discuss future directions for ...
In this case, based on the predetermined fluorescence parameters, an electric charge is applied to the cell of interest using an electrostatic deflection system, and the cell is magnetically isolated.15 The potential limitations of these techniques include the need for a large initial amount of ...
including components and delivery methods. Established delivery methods include DNA, RNA, and peptide vaccines, while newer platforms are being investigated. This section discusses the advantages and limitations of each platform in order to determine the most effective neoantigen-based cancer vaccine delive...
Strengths and limitations of the study A strength of this study is the fact that all interviews were conducted by one researcher who does not work for a Chinese authority or organization, and this may have decreased the pressure on the respondents to give answers perceived as being in line wi...