Chalcone and stilbene synthases (CHS and STS) catalyze condensation reactions of p-coumaroyl-CoA and three C(2)-units from malonyl-CoA, but catalyze different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. Condensing activities of wild-type CHS and STS as well as...
Chalcone and stilbene synthases expressed in eucaryotes exhibit reduced cross-reactivity in vitro. We have previously observed in vitro cross-reaction of CHS and STS overexpressed in Escherichia coli, resveratrol production by CHS and chalcone production by STS. When expressed in eucaryotic cells, or ...
Tropf S, Kärcher B, Schröder G, Schröder J: Reaction mechanisms of homodimeric plant polyketide synthases (stilbene and chalcone synthase). A single active site for the condensing reaction is sufficient for synthesis of stilbenes, chalcones, and 6′-deoxychalcones. J Biol Chem 270: 7922–...
Results in the last few years have shown that the well-knownchalconesynthase(CHS) is only one example from a family of plantpolyketidesynthases. Other members of the family that are identified by function and sequences are thestilbenesynthases (STS),acridonesynthase (ACS), and apyronesynthase ...
Suh D-Y, Fukuma K, Kagami J, Yamazaki Y, Shibuya M, Ebizuka Y, Sankawam U (2000) Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases. Biochem J. 350: 229-235. Thain SC, Murtas G, Lynn JR, McGrath RB, Millar AJ (2002) The...
Chalcone synthase (CHS) and stilbene synthase (STS) are closely related polyketide synthases which are key enzymes in the biosynthesis of flavonoids and stilbenes. Scots pine (Pinus sylvestris) is an interesting plant for a direct comparison of the enzymes. It not only contains the usual ...
[16]Suh, D.-Y.; Fukuma, K.; Kagami, J.; Yamazaki, Y.; Shibuya, M.; Ebizuka, Y.; Sankawa, U. Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases. Biochem. J 2000, 350, 229–235. ...
pathway (Fig.1)5,12. This promiscuity of CHS catalysis likely decreases the efficiency of flavonoid biosynthesis, but it is also believed to have served as the basis for the evolution of other plant-specific PKSs, including stilbene synthase (STS)14andp-coumaroyltriacetic acid synthase (CTAS)...
An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases. Chem Biol. 2004;11(9):1179–94. Article CAS PubMed Google Scholar Yi J, Derynck MR, Chen L, Dhaubhadel S. Differential expression of CHS7 and CHS8 genes in soybean. Planta....
Two classes of enzymes, stilbene synthases and chalcone synthases, play important roles in the biosynthesis of plant phenolics, such as the conversion of the phenylpropanoid-CoA esters into stilbenoids and flavonoids, respectively. In this study, we investigated the transcriptional responses of the ...