Transfer to PVDF membrane at 30 volts for 1 hour (refer to Life Technologies' manual). Block with 20 ml 3% BSA in PBST at 25 C for 1 hour. For anti-J-chain Western blot, add anti-J (SP105, Thermo Fisher) at 1:500 in 3% BSA in PBST overnight at 4° C. Wash with PBST four...
The Treaty of Lisbon, rather than introducing substantial modifications to the policy’s structure, transposed pre-existing provisions into the new Treaty on the Function of the European Union (TFEU) (formerly Art. 32 TEC, now 38 TFEU). Consequently, the specific characteristics of the CAP, the...