cDNA of bovine cGMP-dependent protein kinase (cGMP kinase) isozymes Iα and Iβ differ only in their amino-terminal domains (amino acids 1–89 and 1–104, respectively). Each recombinant isozyme (rIα and rIβ) was transiently expressed in COS-7 cells and its properties were compared with...
In the present work, we demonstrate expression of DT-2 sensitive, cGMP-dependent kinase activity that comigrates with PKGI immunoreactive protein in RN46A cells, where we also document colocalization of SERT with PKGI, but not PKGII, by quantitative immunofluorescence approaches. SERT/PKGI co...
cGMP-Dependent Protein Kinase (α-Isozyme),货号:普洛麦格 Promega V5171,6,000u,SIG,*,大量现货,*! 详情介绍 "产品名称:cGMP-Dependent Protein Kinase (α-Isozyme)品牌及货号:Promega V5171关键词:cGMP-Dependent Protein Kinase (α-Isozyme)cGMP-Dependent Protein Kinase (α-Isozyme) 其他相关产品:"Prote...
(1989). Molecular cloning and predicted full-length amino acid sequence of the type I beta isozyme of cGMP-dependent protein kinase from human pla- centa. FEBS Lett 255,321-329.Sandberg M, Natarajan V, Ronander I, Kalderon D, Walter U, Lohmann SM, Jahnsen T (1989) Molecular cloning and...
Autophosphorylation of purified bovine Ibeta isozyme of cGMP-dependent protein kinase (Ibeta cGK) in the presence of cGMP or cAMP increased basal kinase activity (-cGMP) as much as 4-fold and reduced the Ka for both cGMP and cAMP; maximum catalytic activity (+cGMP) was not altered. ...
Examples of serine/threonine kinases are the cGMP-dependent protein kinases (PKG, cGK, respectively) and the cAMP-dependent protein kinase (PKA, cAK, respectively). PKG is mainly activated by the cyclic nucleotide cGMP. In eukaryotes, two different genes were identified: prkg 1 coding for PKG-...