虽然Gal4模式在酵母菌科分支中已经得到了很好的描述,但对其他酵母(比如致病性模式生物白念珠菌)中GAL通路的调控知之甚少。在该研究中,作者发现Rep1,一个Ndt80样家族转录因子,在共生致病性真菌白色念珠菌中作为半乳糖传感器。它出现在GAL基因启动子上,独立于半乳糖的存在。Rep1通过直接的物理相互作用来识别半乳糖,但...
GAL4UASNAD目的:利用GAL4/UAS基因表达调控系统研究CG9940基因过表达对果蝇心脏抗衰老功能,运动能力和寿命的影响.方法:W1118雌性果蝇与arm Gal4雄性果蝇杂交取F1为对照组,y[1]w[67c23]P{y[+m8] =Mae UAS.6.11}CG9940[GG01267]雌性果蝇与arm Gal4雄性果蝇杂交取F1为过表达组,2组均收集处女蝇1200只,在第1...
结果表明,与对照组果蝇比较,在生殖细胞和包囊细胞中缺失CG8005基因,雄性果蝇的生育能力降低甚至完全丧失;另外,nos-gal4驱动UAS-CG8005 RNAi的雄性果蝇中生殖细胞及生殖融合体消失,并且生殖细胞增殖能力减弱,而tj-gal4驱动UAS-CG8005 RNAi的果蝇睾丸中出现生殖细胞样肿瘤;在果蝇S2细胞中敲减CG8005基因导致细胞凋亡增加...
Neuronal silencing of dendoU expression severely impairs fly lifespan, development and motor performance. Since eyes and brain are the major components of Drosophila's head, where dendoU is predom- inantly expressed, we used the developing eye-specific GMR-GAL4, or the pan-neuronal elav-...
周期蛋白依赖性激酶CABLES2抗体二氯烯胺Porcine GAL4 (Galectin 4) 兔抗鸡IgY抗体二氯烯胺Porcine AVPR1A (Arginine Vasopressin Receptor 1A) 20号染色体开放阅读框128抗体2-氯-6-氟苯基乙酸Porcine Bid (BH3 Interacting Domain Death Agonist) 色素P450ⅡE1抗体2-氯-6-氟苯基乙酸Porcine C3c (Complement Fragment...
The Cg Gal4 was used to express the transgenes in the hemocytes. Virgin females of CgGal4 were crossed with males of the UAS-HttQ103-GFP line. The GFP positive virgin females from the progeny were next crossed with the males of two test chaperone lines, UAS CG-5001 and UAS P58IPK,...
Fig. 4: Somatic CG6015 inactivation leads to aberrant dpERK expression. Full size image Dsor1 is required for CySC maintenance and GSC differentiation in CySC lineages Fig. 5: Somatic Dsor1 is indispensable for CySC maintenance and GSC differentiation. ...
P/CyO、ap—Gal4/BCG,Jwhszflp;FRT2Aubiquitin-GFP/TM6B、hs-flp:FRTGl3 u biqu#in—GFP/Cyo均山本实验宦保存。 2.1.2抗体及其他试剂 212l抗体披遗传学试剂 I PBS溶液(氯化钠、氯化钾、十:水磷酸氢钠、磷酸,氢窜{J.北京化 Tj) 0I%PBST溶液(I×PBS+OI%Triton—100) ...
12Xo3cvCPHKtTmedWz1tAiBchzgaL4X6Ef 0.00961939BTC 3JX2zh1a1JD3VYqmqmoZbJ35QcAcZX1dDQ 0.00381758BTC 1E2PyJt6MMd7uLMBtyD1ot5MLMHAFRTwBV 0.01105277BTC 3EB6GmXSvLmsEspzK42rS9ieze3rrnN3TE 0.01672937BTC 1MWMfaQvcy3xPVsuTgwhp3gCsVf9BCPmCJ 0.01003763BTC 1ADuVmYJfxhQ2aQy38cx9RTPHVe7VVbJ1M 0.0...
Glucose repression of lactose/galactose metabolism in Kluyveromyces lactis is determined by the concentration of the transcriptional activator LA1C9 (K1GAL4) In the budding yeast Kluyveromyces lactis glucose repression of genes involved in lactose and galactose metabolism Is primarily mediated by LAC9 (...