Although the log determinants were quite similar, Box’s M test indicated that the assumption of equality of covariance matrices was violated. Consequently, a quadratic discriminant analysis was chosen. Table 3. Classification of the two experimental groups (low and high cutting) using discriminant ...
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cDNA was then synthesised from 500 ng RNA template using one-step gDNA removal and cDNA synthesis SuperMix, following the manufacturer’s instructions. 2.5.2. Quantitative real-time PCR The time-dependent and tissue-specific mRNA expression of the Sitotroga cerealella JH and ecdysone pathway genes...