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Co-Immunoprecipitation (Co-IP) Assay Cells were infected with flag-CEP55 for 48 h, and then cells were lysed with cold lysis buffer (1% Triton X-100, 40 mM Hepes pH 7.5, 120 mM NaCl, 1 mM EDTA, 10 mM pyrophosphate, 10 mM glycerophosphate, 50 mM NaF, 0.5 mM orthovanadate, PMSF, ...
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