What is the advantage of using an internal standard in a gas chromatography experiment? 1. What does heat and/or acid do to proteins like casein? 2. What would be the purpose of adding calcium carbonate after the removal of the casein, and what would you produce? 3. Explain the purp...
An experiment may be judged successful merely if two or more kinds of particles have been separated; we then may not care to know the exact parameters of the gradient that brought about that happy juncture. If, however, we need to know the equilibrium densities of separated particles, or wis...
Each experiment was conducted three times. The EGFP plasmid was transfected into Hep3B cells using three different methods: Lipofectamine™ 2000 transfection, the PEI method optimized in the previous section, and the PEI method optimized in the previous section with plate centrifugation at 1000 × ...
S3). To minimize this effect, an assembled microfluidic chip that was exposed to O2 plasma after two days was utilized in the experiment. Fluid flow was controlled by the rotation speed of the developed heater stage. Prior to microbead filling, the channels were blocked with 1% BSA. After ...
Considerations When Selecting Conical Tubes for Centrifugation ApplicationsThermo Scientific CentrifugeModel LegendTM 1XR Legend 1XR Legend 1XRStephanie CarterWenxiao LuMarci MooreJoe GranchelliCindy Neeley
were solubilized in 100 μL of a solution made of 100 mM Tris-HCl (BioRad Laboratories) pH 8.8, 10 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl), 50 mM trehalose (Acros Organics), final pH 7.0 at RT from 15 minutes up to 1 hour depending on the experiment. ...
Lab-on-a-disc: Sorting out tumor cells Simultaneous isolation and quantification of individual cells and cell clusters is possible thanks to a centrifugal sedimentation approach. Tumor cells circulating in the blood are an excellent indicator for the prognosis of various cancers. In particular, the ...
In the experiment, the centrifuge (Centrifuge 5417 R, Eppendorf, Germany) used in our droplet-generating device has a flux of 12 rotors, which can achieve the simultaneous operation of 12 droplet devices. By altering the model of the centrifuge, the droplet generation scale can be expanded ...