Disinfection and supply center is the core Department of the hospital, which undertakes all reusable diagnostic and therapeutic instruments, instruments and articles cleaning, disinfection, sterilization and supply of sterile articles. It is also the log
A 5-ml syringe filled with ice-cold PBS (Gibco, 10010049) was inserted at the distal-most end of the spinal column, and steady pressure was applied to extrude the spinal cord into a 100-mm Petri dish filled with sterile PBS on ice. The lumbar segments of the spinal cord tissue were c...
After sterilization, 33.3 mL or 100 mL of the 60 % stock glucose solution (SC-2 or SC-5, respectively), 20 mL of the 50x stock MES buffer solution, and sterile dH2O were added to adjust the total volume to 1.0 L. Yeast Synthetic Drop-out medium supplements (SIGMA) ...
The cell suspension was transferred to sterile, disposable electroporation cuvettes with a 4-mm electrode gap (Molecular BioProducts, San Diego, CA). The cells were subjected to two square 400-V pulses of 50 µs each from the PA-4000 PulseAgile genera- tor. Following electroporation the ...
These personnel, however, are more likely to make operational errors owing to their limited experience with the imaging system, potentially requiring the surgeon or their assistant to leave the sterile operating area so that they can operate the computerized imaging systems directly, after which re-...
BAL samples were obtained as previously described, using two washes of 40-ml sterile saline 2 weeks before vaccination and at 3, 7, 11 and 14 weeks20,24,25,29,32 and analysed for CFUs. Necropsy to collect tissues was performed during euthanasia. There were two different reasons for ...
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Biofilm was removed by brushing the rocks in sterile plastic containers with a sterile toothbrush to loosen any firmly attached material. Once the water was saturated with biofilm, it was filtered to remove sand, invertebrates, and other flotsam that was also attached to the rocks by pouring ...
Each treatment was removed from the container and macerated in liquid nitrogen for processing. RNA extraction For each treatment, three biological replicates were used (three banana plants). A total of 45 corresponding sam- ples were macerated in liquid nitrogen and transferred to 2 ml sterile ...
Porcine small intestine and duodenum samples were collected from Woori Bio-Food & Bio-Tech Co., Ltd. (Iksan, Korea). First, 1 g of porcine small intestine and duodenum samples were serially diluted in sterile PBS buffer (pH 6.8). Then, 100 µL samples were smeared onto the surface of...