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Using the draw tool in Fiji, a circle encompassing the GSC was drawn and the integrated fluorescence density (IFD) was taken and background subtracted (IFD - (average mean intensity of background x area of the region sampled)). All results were then normalised to controls. Quantification of ...
Summerson: We did have some SkyPanels with us that we used, but we also gelled old school HMIs and Fresnels, so there was a mix. I come from a lighting background, so I started out in lighting before there were LEDs. I love LEDs, but the heads are quite often...