该技术最初用于研究药物在哺乳动物细胞中的结合,但现在广泛用于验证和量化药物在不同物种的细胞和组织中的靶点接合。 具体而言,该方法通过将目标细胞和药物在不同温度下共培育一定时间后裂解,与药物结合的蛋白会因为热稳定性提高而更不容易发生变性而沉淀,随后通过离心把沉淀的未结合蛋白去除,最后通过蛋白免疫印迹法(We...
揭示生命机制的热力钥匙：Cellular Thermal Shift Assay（CETSA）的深度探索CETSA，这一生物物理技术的璀璨明珠，自2013年问世以来，以其独特的洞察力在细胞生物学领域崭露头角。它聚焦于药物与细胞内蛋白的互动，尤其在药物靶向性和脱靶效应的评估中发挥着关键作用。通过在不同温度下培育细胞与药物，CETSA揭...
卡罗林斯卡研究中心科学家开发出一种称之为细胞热转移(CETSA,Cellular Thermal Shift Assay)的方法(Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay, D Martinez Molina, R Jafari, M Ignatushchenko, T Seki, E.A Larsson, C Dan, L Sreekumar, Y Cao, P No...
有没有了解细胞热转变分析（Cellular Thermal Shift Assay，缩写：CETSA）这个技术的大神啊？？请教这个...
cellular thermal shift assay (cetsa) for determining the drug binding affinity using ba/f3 clones stably expressing receptor pseudokinases author links open overlay panel hanna karvonen a † , juuli raivola b , daniela ungureanu a b c show more add to mendeley share cite https://doi.org/...
基于cellular thermal shift assay (CETSA) 的高通量药物靶点筛选 http://t.cn/z87CuXK
Cellular thermal shift assay (CETSA) The ability of compounds to interact with, and thereby stabilize the target in intact cells, was analysed essentially as described by Molinaet al.21. Detailed protocol is provided in theSupplementary materials and methods. ...
In this way, The cellular thermal shift assay for evaluating drug target interactions in cellsRozbeh Jafari 1 , Helena Almqvist 2 , Hanna Axelsson 2 , Marina Ignatushchenko 1 , Thomas Lundbäck 2 , Pär Nordlund 1 & Daniel Martinez Molina 11 Department of Medical Biochemistry and ...
Recently, we published a proof-of-principle study describing the implementation of thermal shift assays in a cellular format, which we call the cellular thermal shift assay (CETSA). The method allows studies of target engagement of drug candidates in a cellular context, herein exemplified with ...