2B). We further analyzed the expression of the protein P53, a marker of cellular senescence, in whole-cell lysates of NSCsWT from passages 1, 3 and 5. While NSCs−/− didn’t express P53 throughout the 5 pass
Senescence is a cell’s fate elicited in response to external and internal cellular stress signals, established through transcription factor cascades that can include p16INK4a (p16)/retinoblastoma protein and/or p53/p21CIP1 (these factors are thought as the markers of cellular senescence). ...
Caspases also have a role in T cell proliferation. The levels of caspase-3 and caspase-8 are associated with the timing of activated mouse T cells entering the proliferative phase, as indicated by the expression Ki67. In addition, the use of caspase inhibitors suppresses T cell proliferation [...
a number of researchers have adopted siRNA methodologies to assist in loss of function studies by targeting H2S biosynthetic enzyme expression levels. These techniques have been particularly amenable for use in cell-culture systems. As shown in Table1, these approaches have assisted researchers in the...
Cellular senescence can act as both tumor suppressor and tumor promoter depending on the cellular contexts. On one hand, premature senescence has been considered as an innate host defense mechanism against carcinogenesis in mammals. In response to variou
Cellular senescence represents a permanent shift in a cell’s phenotype to an aged phenotype, likely caused by mechanisms involving one or multiple other aging hallmarks. Phenotypically, senescent cells exhibit increased expression of p16INK4a and p21 following the activation of either the p53-p21 ...
Importantly, this knowledge has enabled a better understanding of the molecular basis of a number of diseases caused by mutations in the genes encoding m5C methyltransferases or changes in the expression level of these enzymes. Keywords: RNA methyltransferase; RNA modification; epitranscriptome; 5-...
for the aberrant expression of liver MAdCAM-1.125Cysteamine and other amines derived from gut bacteria and diets can enter the liver via the portal vein and act as substrates for VAP-1, a potent amine oxidase, leading to the generation of catabolites that induce MAdCAM-1 expression on HSECs...
knockdown of cyclin D1 induced oxidative imbalance by elevating intracellular ROS levels in cancer cells, which promoted the senescence of cancer cells through a retinoblastoma-independent pro-senescence pathway [138]. These investigations suggest that anti-breast cancer therapy targeting cyclin D1 could...
Since their establishment in the early 1970s, the nuclear changes upon apoptosis induction, such as the condensation of chromatin, disassembly of nuclear scaffold proteins and degradation of DNA, were, and still are, considered as the essential steps and hallmarks of apoptosis. These are the charac...