senescent LFs when exposed to alveolar epithelial cells (AECs) in vitro.147This study offers a possible explanation for the abnormal abundance of senescent cells in the lungs of patients with idiopathic pulmonary fibrosis. We propose that senescent cells, including chondrocytes and other bone-resident...
To further elucidate the impact of glycolytic flux and mitochondrial respiration to energy production, we calculated the ECAR to OCR ratio and visualized the total levels by plotting the ECAR against the OCR (Supplementary Fig. 2d–f). The higher the ratio, the lower the proportion of mitochondri...
Strikingly, proteins related to ribosomes and translation were downregulated with increasing ploidy. The observed PDR of ribosome biosynthesis and translation also provided an explanation for the allometric proteome scaling, which we propose is regulated by the mTORC1-Sch9-Tup1 axes (Fig.5j). Tup1 ...
OCR was used to calculate basal respiration, ATP-coupled and uncoupled respiration and spare respiratory capacity; glycolysis and glycolytic reserve were calculated from ECAR. Results are presented as ± SEM, n = 3 independent experiments; unpaired t-test Full size image...
mitochondrial dysfunction in spinal motor neurons, with decreased basal and maximal mitochondrial respiration, impaired mitochondrial membrane potential, impaired mitochondrial mobility, increased oxidative stress level and increased fragmentation [179]. Interestingly, mitochondrial defects in SMA are not thought ...
We therefore asked how SFXN1 KOs are able to maintain cellular baseline respiration. We observed higher glucose use, with largely unaltered reliance on glutamine and fatty acid pathways (Figure 5D). It remains a possibility that SFXN1 KOs were able to switch to other fuels not tested in this...
Moreover, the reaction of NO• with , which is formed by the mitochondrial respiratory chain, leads to the switch from reversible inhibition of cellular respiration by NO• to the pathological inhibition of mitochondrial function by ONOO− [94]. 3. Role of ROS Sources in Oxidative Stress...
Perturbation of CL synthesis alters mitochondrial bioenergetics, resulting in reduced membrane potential, inefficient coupling of respiration, and decreased ATP synthesis (3, 4, 5, 6). In the inner membrane, CL is tightly associated with several proteins in respiratory complexes I, III, and IV (7...
9C) and a decrease in maximal respiration rate in the L310S line (adjusted p-value 0.0201) (Fig. 9D). These results highlight that, relative to the control cell line, the loss-of-function line had a positive change in energy utilization and improved mitochondrial capacity, whereas the ...
The mitochondrial mRNA-stabilizing protein SLIRP regulates skeletal muscle mitochondrial structure and respiration by exercise-recoverable mechanisms Tang Cam Phung Pham Steffen Henning Raun Lykke Sylow Nature Communications (2024) Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures ...