Induction of spermatogenesis from FGSCs to haploid spermatid-like cells in vitro On the basis of the above results, we investigated whether FGSCs have the potential to transdifferentiate into SLCs in vitro. FGSCs isolated from actin-GFP mice were cultured for eight passages, mixed with Kitw/wv...
Prior studies have shown that PC cell lines cultured for an extended time without androgens tend to display neuroendocrine-like phenotypes20,21. The largest fold changes in chromatin accessibility based on average signal from all cells showed over representation for neuronal system processes between the...
At a time when mainstream international theory seems to discover the value of conventional international law as a form of regulation of the global system, it can be interesting to revisit one of the most classic debates in the field of international norms. We are talking about international , ...
Human embryonic stem cells (HuES-7; HuES-9) and human induced pluripotent stem cells (adult dermal fibroblast iPS; neonatal foreskin fibroblast iPS) were cultured without feeders on Matrigel (Corning, #354277) for 1–3 passages before starting the experiments. 1:1 mixture of conditioned and un...
PRELIMINARY STUDY ON FORMATION OF ECCRINE SWEAT GLAND-LIKE STRUCTURE IN THREE-DIMENSIONAL CELL CULTURE sweat gland epithelial cells were cultured in vitro and were observed under inverted phase contrast microscope.These cells at the density of 2 脳 105/cm2... L Zhou,WU Jinjin,X Lei,... - 《...
Sciatic nerves from 1～3 days old SD rats were taken out . The Schwann cells were cultured and purified by modified repeated explanation, and fibroblastic cells were removed by rapid trysinization , differential adhesion and anti mitosis methods. The Schwann cells were identified by indirect ...
ART can induce radiosensitivity of HeLa cells in vitro and in vivo.Introduction Cervical cancer used to be the number 1 killer of female human beings who suffered from cancer, with the development of general survey technique, the morbidity of cervical cancer decreased recently. However, cervical ...
From the initiation of primordial follicular formation to the growth of early secondary follicles, ovarian tissues from 16.5 dpc fetal mice were cultured in vitro for 14 days. Subsequently, 678 intact secondary follicles were isolated from 182 mouse fetal ovaries and cultured for 12 days. A total...
Cadaveric human islets used as control were obtained from Tebu-bio (Magenta, Italy). In Vitro Differentiation of HLSC into Islet-like Structures (HLSC-ILS) HLSC were cultured at a density of 1.3E + 4/cm2 in a medium consisting in RPMI 1640 supplemented with 10% Fetal Bovine Serum...
All cells were cultured in a humidified atmosphere with 5% CO2 at 37 oC respectively. Colony formation assay To evaluate the potential of self-renewal of the AS cells, the colony formation assay was carried out using a reported methodology28. Cells were cultured to 70–80% confluence and ...