Invitrogen Cells-to-CT 试剂盒通过 实时荧光定量 PCR (RT-qPCR) 测量相对基因表达,无需在扩增前进行 RNA 纯化。Cells-to-CT 试剂盒采用 TaqMan 和 SYBR Green 检测化学试剂,为直接使用培养细胞进行 qPCR 分析提供完整的工作流程,为您提供出色的性能、可靠性和世界一流的支持。...
Invitrogen Cells-to-CT 试剂盒通过 实时荧光定量 PCR (RT-qPCR) 测量相对基因表达,无需在扩增前进行 RNA 纯化。Cells-to-CT 试剂盒采用 TaqMan 和 SYBR Green 检测化学试剂,为直接使用培养细胞进行 qPCR 分析提供完整的工作流程,为您提供出色的性能、...
Cells were immunostained with anti-SOX9 (GMPR9; Thermofisher, Waltham, MA, USA), anti-aggrecan (Santa Cruz sc-33695) and Anti-Collagen II (NBP2-46,876; NovusBio, Littleton, CO, USA), antibodies, followed by DAPI (Life Technologies, Carlsbad, CA, USA). Cells were visualized with a ...
Complementary DNA corresponding to 5 ng RNA was used for real-time PCR performed by QuantiFast SYBR Green PCR Master Mix (Qiagen) on the 7500 Fast real-time PCR system, 7500 software v.2.0.6 (Applied Biosciences by Thermo Fisher Scientific). Primers for the specific target amplification ...
Human HEK293T, A375 and A2058 cells was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Massachusetts, USA). Other cells were cultured in RPMI1640 medium (Gibco). All mediums are supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and...
eFluor 450 anti-mouse CD11b antibody Thermofisher Cat# 48-0112-82; RRID:AB_1582236 BV510 anti-mouse NK1.1 antibody BioLegend Cat# 108737; RRID:AB_2562216 PE-Cy7 anti-mouse NKp46 antibody BioLegend Cat# 137617; RRID:AB_11218594 PE-Cy7 anti-mouse Ly6C antibody Invitrogen Cat# 25-5931-...
DMEM/F12 ThermoFisher Cat# A4192001 DNase Beyotime Cat# D7076 RNase Beyotime Cat# ST576 Spontaneous fluorescence quencher Servicebio Cat# G1221 Protein A/G Magnetic Beads MedChemExpress Cat# HY-K0202 Resource availability Lead contact Further information and requests for resources and reagents should...
The quantification and quality control of extracting RNA was conducted in triplicate with a NanoDrop spectrophotometer (Thermo Fisher Scientific, Germany). Then, RNAs were reverse-transcribed using the RevertAid First Strand cDNA Synthesis (Thermo Fisher Scientific, Germany). The quantitative RT-PCR ...
Perform RT-PCR directly from cell lysates without extraction using the newest product in the Cells-to-CTfamily. This kit allows you to prepare cells for RT-qPCR analysis in just 5 minutes with a simplified workflow that is suitable for a few samples, or scalable ...
What is Cells-to-CT? Invitrogen Cells-to-CT kits measure relative gene expression by real-time quantitative PCR (RT-qPCR) without the need for RNA purification prior to amplification. Available in both TaqMan and SYBR Green detection chemistries, Cells-to-CT ...