Hematopoietic stem cells (HSCs) produce highly diverse cell lineages. Here, we chart native lineage pathways emanating from HSCs and define their physiological regulation by computationally integrating experimental approaches for fate mapping, mitotic tr
Using this transition matrix, we set out to study gene dynamics and fate choice among ABpxp cells. As a first step, we use moslin/CellRank 2 to compute seven terminal states and recover known ciliated-neuronal, non-ciliated-neuronal, glia, and excretory subtypes [7] (Fig.3d). The termina...
In a final step, counts were smoothed by the mean expression across their neighbors to compute final RNA abundances. These steps were performed by scVelo’s moments function. To estimate RNA velocity, the preprocessed unspliced and spliced abundances were (gene-wise) min−max scaled to the ...
Cell fate decisions are fundamental for development, but we do not know how transcriptional networks reorganize during the transition from a pluripotent to a differentiated cell state. Here, we asked how mouse embryonic stem cells (ESCs) leave the pluripotent state and choose between germ layer fat...
a2.We want to decide our own fate 2.We想要决定我们自己的命运[translate] aThe prognostic impact of age in patients with triple-negative breast cancer. 年龄的预断冲击在病人有三倍消极乳腺癌。[translate] aa subject most neurobiologists considered off-limits. 多数神经生物学家认为不允许去的主题。[tra...
These measures based on the number of mis- segmented voxels have the advantage of being simple to compute. However, they do not take into account the lo- cation of a mis-segmented voxel. The location might be important since a mis-segmented voxel close to a seg- ment boundary might not ...
Two-way ANOVA and Tukey’s multiple comparison test were used to compute the statistical significance at day 21 postgraft for the different treatment groups. **p ≤ 0.01; ****p ≤ 0.0001. (E–I) Percentages of GFP+ OT-1 control and OT-1 miR-155 in total CD8+ T cells in (E) ...
(Rbpj) is a transcription factor commonly known for its function as a co-factor during Notch signaling, translating extracellular signals into gene expression changes15. In the context of T cell differentiation and function, Rbpj has been associated with TH1/TH2 cell fate decisions16,17. Indeed,...
Gaussian kernel density estimation to compute density of cells from various conditions in the UMAP embedding was performed using Scanpy’s tl.embedding_density. Pre-ranked gene set enrichment analysis (GSEA) was implemented in fgsea (v1.24), using the Wald statistic as the gene rank metric. ...
The epithelial-to-mesenchymal transition (EMT) and the unjamming transition (UJT) each comprises a gateway to cellular migration, plasticity and remodeling, but the extent to which these core programs are distinct, overlapping, or identical has remained