High-power femtosecond lasers can induce damage to cells and tissues by a process called optical breakdown60 that ultimately leads to thermal and mechanical disruption of the cell or tissue to which they are directed. We used this principle to ablate epidermal or tactile bristle cells at the desi...
Autophagy: renovation of cells and tissues. Cell 147, 728–741 (2011). Article CAS PubMed Google Scholar Kimura, S., Noda, T. & Yoshimori, T. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 3, 452–460 (2007). ...
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AGD1, also named as MIR100HG, a subclass of lncmiRHG, encodes three miRNAs within introns: miR-125b-1, miR-100, and let-7a-2. Evidence shows that AGD1 is dysregulated in multiple tumors compared to normal tissues, acting as either an oncogene or tumor suppressor [18]. Previous ...
The MC signature genes were used as MC markers to assess the density of MCs in bulk RNA-seq samples of both tumors and normal tissues. All five MC signature genes that achieved statistically significant (P < 0.05) were considered credible. Results showed that MC signature genes were signi...
Among the commonly sought-after tissues for stem cell extraction, such as adipose tissue and bone marrow, perinatal tissues such as the placenta, amnion, and chorion have proven to be rich sources of stem cells. The stem cells obtained from these perinatal tissues possess highly desirable ...
Serum was then separated from the blood and the concentrations of IL-1β, IL-10 and IL-23 were determined using ELISA kits (Jiangsu Meimian Industrial Co., Ltd, China) according to the manufacturer’s instructions. Rat heart, liver, spleen, lung and kidney tissues were harvested from rats...
post hoc Tukey’s adjustment (Figs.2C,5A, B,6A and7B) or Dunn’ multiple comparison test (7A). Significance was taken atp ≤ 0.05 (*),p ≤ 0.01 (**),p ≤ 0.001 (***), andp ≤ 0.0001 (***). All graphs were made in GraphPad Prism and display mean ±...
4). These data indicated that the tumor NK cell compartment was phenotypically distinct to the ILC1 populations identified in non-tumor tissues, consistent with recent scRNA-seq analyses21. Fig. 2: Differential expression of CD11b and CD49a capture temporal changes in NK cell phenotype. A ...
In this study, we applied SMA at low concentration to human primary cardiovascular cells and rat vascular tissue, to ‘biopsy’ cell proteins while avoiding significant reductions in cell viability. SMA at 6.25 parts per million harvested proteins from cells and tissues without causing significant ...