The cellulase reaction is carried out in the presence of ferricyanide and cellobiose dehydrogenase thus allowing the continuous oxidation of cellobiose produced (or alternatively the cellodextrins that may or ma
This chapter describes the the procedure for the production, partial purification, and characterization of a cellobiose dehydrogenase produced by a species of Monilia. A zymogram method for the detection of cellobiose dehydrogenase activity is also described. Cellobiose dehydrogenase produced by the Monilia...
To further rule out reduction of the NcLPMO9F copper centre by the FAD cofactor in the NcCDH dehydrogenase, we performed a second stopped-flow experiment, which demonstrated conclusively that the NcDH domain alone is unable to reduce Cu(II) in NcLPMO9F (Fig. 4h). Our results demonstrate ...
Microb Cell Fact (2021) 20:2 https://doi.org/10.1186/s12934-020-01492-0 Microbial Cell Factories RESEARCH Open Access Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei Lena Wohlschlager1, Florian Csarman1, Hucheng Chang1, Elisabeth ...
Glutamate Dehydrogenase (NADP+), recombinant from Bacteria [rGIDH] 品牌:O Yeast 康宁corning 3640 PLATE,384 WELL,CLR,NBS,FLUORES 384孔板 平底 透明 非结合表面 用于荧光测定 25个/包 Flumioxazin Standard 丙炔氟草胺标准品 品牌:FUJIFILM Wako 双氰胺(二聚氰胺,氰基胍),Dicyandiamine,[461-58-5],O2si...
Hallberg BM, Henriksson G, Pettersson G, Divne C (2002) Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase. J Mol Biol 315:421–434 Article CAS Google Scholar Harreither W, Sygmund C, Dünhofen E, Vicuña R, Haltrich D, Ludwig R (...
Interference of laccase in determination of cellobiose dehydrogenase activity of Pleurotus ostreatus (Florida) using dichlorophenol indophenol as the electron acceptor[J] . TanimaSaha,Tapas KumarChakraborty,RinaSaha,NirmalenduDas.J. Basic Microbiol. . 2005 (2)...
Neither other carbohydrate oxidoreductases (pyranose dehydrogenase, FAD-dependent glucose dehydrogenase, glucose oxidase) nor laccase interfered with CDH activity in this assay. Applicability of the developed assay for the selection of new ascomycetous CDH producers as well as possibility of the ...
Assay for cellobiose dehydrogenase in the presence of laccase11111 2 3 4 5 6 7 8 9 10111 1 2 3 4 5 6 7 8 9 20111 1 2 3 4 5 6 7 8 9 30111 1 2 3 4 5 6 7 8 9 40111 1 2 3 4 5 6 7 8 9 50111 1 2 3111G. Henriksson†...
SYGMUND C., SANTER P., KRONDORFER I., KRONDORFER I., PETERBAUER C., ALCALDE M., HYANHONGO G., GUEBITZ G., LUDWIG R.; Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production, Microbial Cell Factories, 2013, 12:38...