Here, we discuss the latest advances in cell-free biosensor research and highlight some key challenges to address for the field to bring its full potential.doi:10.1016/j.cobme.2019.08.005Peter L. VoyvodicJerome BonnetCurrent Opinion in Biomedical Engineering...
cell-free biosensorenvironmental monitoringmedical diagnosissynthetic biologyworkflowSynthetic biology has promoted the development of biosensors as tools for detecting trace substances. In the past, biosensors based on synthetic biology have been designed on living cells, but the development of cell ...
A cell-free biosensor signal amplification circuit with polymerase strand recycling Article 13 January 2025 A primer on emerging field-deployable synthetic biology tools for global water quality monitoring Article Open access 03 April 2020 Real-time bioelectronic sensing of environmental contaminants ...
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By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC–MS measurements of...
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P.aeruginosa-Infected Respiratory Samples Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, ... KY Wen,L Cameron,J Chappell,... -...
The cell-free system offers potential advantages in biosensor applications, but its limited time for protein synthesis poses a challenge in creating enough fluorescent signals to detect low limits of the analyte while providing a robust sensing module at the beginning. In this study, we harnessed ...
Using this biosensor, we identified a potential bottleneck in ribosome content. Therefore, we undertook strain engineering to overexpress global regulators of ribosome biogenesis to increase in vitro protein production. CFPS extracts from the strain overexpressing FHL1 had a three‐fold increase in ...
We show the compatibility of different concepts of in vitro synthesis toward biosensor applicability by the example of Cyt-bo3 protein expression. Our results obtained from in vitro synthesized proteins displayed similar behavior to proteins isolated from the cellular context. Overall, our approach is ...
The workflow to engineer a cell-free biosensor detecting a novel molecule is straightforward (Fig.1c). First, possible metabolic pathways to convert the molecule of interest into a detectable ligand are identified using SensiPath. Second, the genes coding for the metabolic transducer enzyme, the ...