Editorial: Cell Free Biocatalysis for the Production of BioproductsBiological approaches show promise for the sustainable and cost competitive production of commodity fuels and chemicals. A lot of efforts have been devoted to engineering microbial chassis to produce select fuels and chemicals. These ...
Cell-free protein synthesis (CFPS) serves as an innovation booster in the field of biocatalysis. By integrating CFPS into the design and development of biocatalysts, the discovery, synthesis, and screening of previously untapped enzymes and their engineered variants can be improved. The high-throughpu...
Here, we report a unified biocatalysis (UniBioCat) system based on cell-free gene expression for rapid biosynthesis and engineering of RiPPs. We demonstrate UniBioCat by reconstituting a full biosynthetic pathway for de novo biosynthesis of salivaricin B, a lanthipeptide RiPP. Next, we delete ...
Synthetic biology and accompanying cell-free biocatalysis have been proposed as a viable approach to overcome many of these limitations. However, cell-free biocatalysis faces its own limitations for industrial applications due to high enzyme costs and cofactor consumption. We have constructed a cell-...
Cell free biocatalysis is showing promise as a replacement or complement to conventional microbial biocatalysts due to the potential for achieving high yields, titers, and productivities. However, there exist several challenges that need to be addressed before its broader industrial adoption is achieved...
展开 关键词: alcohols biocatalysis biosynthesis biotechnology enzymes DOI: 10.1002/cssc.201200365 被引量: 153 年份: 2012 收藏 引用 批量引用 报错 分享 全部来源 免费下载 求助全文 全文购买 国家科技图书文献中心 (权威机构) Wiley Wiley Wiley Wiley (全网免费下载) 查看更多 相似文献 参考文献 引证文献Metab...
Therefore, the combination of multifactor protein engineering and biocatalysis exhibits enormous potential not only to enhance the intrinsic catalytic activity and stability of enzymes but also to maximize the performance of spatially organized multi-enzyme systems48. The controlled interactions with key ...
Liyuan.ZhangKey Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.Wei.GuoDepartment of Ecology, Shenyang Agricultural University, Shenyang, Liaoning Province, 110866, China.Yuan.Lu...
We do not provide a comprehensive discussion of in vitro synthetic biology projects, nor do we cover the rich history of in vitro biocatalysis (Zaks, 2001), but rather focus on a description of several recent multi-component examples in the context of metabolite production. Finally, we examine...
By contrast, enzymatic biocatalysis in cell-free biosystems has numerous advantages in production of chemicals with high purity, whereas efficient enzymatic ... Cui, ZhenzhenZhao, YujiaoMao, YufengShi, TingLu, LingxueMa, HongwuWang, ZhiwenChen, Tao - 《Journal of Chemical Technology & Biotechnology...