Cell viability was determined with 0.4% Trypan blue (Sigma-Aldrich, Canada) staining and calculated from the following formula: Percent cell viability equals the number of unstained (living) cells divided by the total number of cells times 100 [58]. Human primary MM samples Bone marrow as...
Viability (percentage with respect to controls) of printed cells on different printing substrates: (A) 4 h after printing; (B) at various times after printing. As control, a defined volume of Living Ink (corresponding to approximately 40,000 cells/well) was withdrawn from the cartridge ...
at 490 nm. The optical density (OD) was used to determine the percentage of viable cells via the following formula: cell viability (%) = (OD treatment group–ODblank group/ODcontrol group–ODblank group) × 100%. Enzyme-linked immunosorbent assay RAW264.7 cells were seeded into a...
The percentage of cells surviving the 5 Gy of ionization radiation in each cell line was calculated as follows: % Cell viability = [A600 (t) − A600 (0)]5 Gy/[A600 (t) − A600 (0)]0 Gy × 100. For examining the effect of BMS345541 (Sigma, 401480) treatment on cell...
In comparison to carprofen, mavacoxib exhibited superior inhibition of cancer cell viability with IC50 values 3.5-fold to 1.5-fold higher, depending on the cell line. Interestingly, neither drug induced apoptosis in normal cells (CPEK). Whereas, in the osteosarcoma cell lines tested, both ...
In the formula, pi represents the percentage of positive cells in the slide; i represents the intensity of A3B staining. Cell culture The adult retinal pigment epithelium cell line ARPE-19 was obtained from the Cell Bank/Stem Cell Bank (Chinese Academy of Sciences). Human cutaneous melanocyte ...
cell migration, invasiveness, proliferation, cell adhesion and the effect of chemotaxis and cytotoxicity in real time [46,47]. No staining of the cells at the end of the experiment is needed. Neither the applied electric potential nor the gold microelectrodes affect the viability and adhesion of...
Cell viability and density were measured using trypan blue staining and by counting the number of cells using a hemocytometer. The single-cell suspension (>1000 cells/µL, cell viability > 90%) in PBS with 0.1% BSA was used for single-cell RNA sequencing (scRNA-seq). 4.3. Single-Cell ...
Cell viability was determined by the MTT-based assay using Cell Proliferation Reagent WST-8 (GenScript, Piscataway, NJ). Cells (4000–5000 cells/well, except WI-38 was 10,000 cells/well) were plated in 96-well culture plates, and serially diluted testing compounds were added to the cells ...
(Fig.3c), without affecting the cell viability (Fig.3aand Supplementary Fig.6f) while HSB-2 cells show induction of apoptosis already after 16 h of dasatinib treatment (Supplementary Fig.6g). Thus, our data suggest that LCK and SRC are highly active in T-ALL, but the pharmacological ...