The diffusion limit of oxygen and nutrients in nonvascular tissues is generally 100–200 μm, which can significantly affect regular cellular behaviors such as cell division, migration, and death [144,145]. Hence,cell viabilityis one of the most important indicators forvascular tissuefunction. Howev...
Each distribution is first normalized according to the average cell viability of the esiLuciferase control (which corresponds to 100%). The two normalized distributions are statistically significantly different if their Mann–Whitney U-test p value is less than or equal to 5%. Gene expression-based...
Samples with a cell viability rate of greater than 80% were subsequently used for single-cell library construction. The entire procedure took approximately 3 h to complete. 10 × library preparation and sequencing The single-cell suspensions were adjusted to a concentration of 1500 cells/µ...
Cell labeling refers to the visualization and tracking live cells over time without compromising their viability, behavior, and functionality. While NPs have been under investigation for cell tracking for roughly two decades, the surge in more innovative tracking methods has been particularly pronounced ...
9 ratio was added to each well. Subsequently, the cells were cultured at 37 °C for a duration of 3 h. Following the incubation period, the absorbance of each well was quantified at 450 nm using a microplate reader (Bio-Rad, CA, USA) to determine the level of cell viability or ...
Cell viability To determine cell viability, MEL290 cells were seeded in 96-well plates at a density of 1000 cells per well and treated with 2.5 nM BAY-1895344 (Selleck, S8666) or 2 μM VE-822. After incubation with 10 μL CCK-8 reagent (Dojindo Laboratories, Kumamoto, Japan) ...
The Countess instrument has been validated for use with more than 20 common cell types, including primary cells, blood cells, yeast cells (without viability assessment), insect cells and fish cells. The Countess automated cell...
[31]. These authors demonstrated that the synergistic effect of temsirolimus with vorinostat on cell viability, colonogenic survival inhibition and apoptosis induction was due to the synergistic inhibition of survivin expression [31]. Similar results and observations were derived by using the survivin ...
cell viability was assessed with Cell Counting Kit-8 (CCK-8) according to the protocol. For colony formation assays, 500–1000 cells per well were seeded in six-well plates and cultured for 2 weeks. Then, colonies were fixed with 100% methanol for 20 min and stained with 0.5% crystal...
Continuous rounds of division, however, create increased reliance on other cell cycle control mechanisms to prevent catastrophic levels of damage and maintain cell viability. New detailed insights into cell cycle control mechanisms and their role in cancer reveal how these dependencies can be best ...