另外,Cell-Tracker Deep Red的激发和发射光谱(630/650nm)与绿色荧光蛋白GFP、红色荧光蛋白RFP都能很好的分开,适用于多重标记(见附表1. Cell-Tracker荧光探针的光谱特征)。 产品特性 1) 同义名:CellTracker™ Deep Red Dye; CellTracker™深红色染料;...
Cell tracker dyes provide the simplest processes for monitoring cell movement and localization. These dyes freely pass through the membrane of cells where they become cell-impermeant by reacting with thiol groups, except for CellTracker Deep Red which reacts with amine groups on proteins. Cell trac...
CellTracker Deep Red—630/660 nm Fluorescent CellTracker reagents include the blue-fluorescent chloromethyl derivatives of amino-, hydroxy-, and difluorohydroxycoumarin (CMAC, CMHC and CMF2HC), the green-fluorescent chloromethyl derivatives of fluorescein diacetate (CMFDA) and a BODIPY™ dye, the ...
CellTracker Deep Red dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission ...
可以选择我司提供的活细胞示踪三色组合试剂盒(绿色/红色/深红色)(Cell-Tracker Three-color Trial Kit (Green/Red/Deep red))(#:MX4111-1Kit),试剂盒提供三种颜色的Cell-tracker探针(Cell-Tracker Green CMFDA、Cell-Tracker Red CMTPX、Cell-Tracker Deep Red),以最小包装形式供应,以最小的成本进行实验条件的...
To induce AICD, activated T cells were stimulated with anti-CD3 (10 μg/mL; BD Bioscience), and tumor-specific CTLs were treated with anti-CD3 (10 μg/mL; BD Bioscience) or autologous tumor cells pre-dyed by CellTtracker Deep Red Dye (Thermo Fisher Scientific) at a 1:1 ratio ...
CytoTraceTM Red Fluorescent Probe细胞质红色荧光探针也叫CytoTrace™ Red CMTPX,和CellTracker Red CMTPX有相同的化学性质。CytoTraceTM Red Fluorescent Probe可以轻易穿透活细胞膜进入细胞内,转变成不具细胞渗透性的反应产物,发出较强荧光信号。随着细胞的传代增殖
Splenocytes of WT and Tlr3/7/9−/− donor mice were isolated and labelled with 1 μM CellTracker Deep Red Dye (Thermo Scientific). Labelled splenocytes (10 × 106) were injected intravenously into recipient mice. One day later, tumours were dissected and cleared. Infiltration of ...
活细胞使用 MitoTracker DeepRed(终浓度500nM) 在暗室中 37℃ 孵育 30min,然后使用 PFA(3.2% vol/vol)细胞固定反应 20min。在室温下使用 triton-100(0.1%)孵育细胞 20min。染色液配置成以下的浓度:5 µL/mL phalloidin, 100 µg/mL conca...
K562 cells ectopically expressed_CD19 and mCherry cells were used as target cells, and CD19- K562 cells labeled with CellTracker Deep Red Dye (Invitrogen, Cat#C34557) were used as non-target cells. The target and non-target cells were mixed at a 1:1 ratio and then co-incubated with ...