CellTrace™ Violet 细胞增殖试剂盒适用于体外和体内标记细胞,采用染料稀释法通过流式细胞分析追踪多代细胞。•卓越的性能—明亮的单峰染色可实现多代细胞可视化•信号保留时间长—染色后染料可在细胞中保留数天•通用—多种颜色可供选择,易于与细胞功能抗体或标志物(如 GFP)结合使用•简单而可靠的染色方案查看...
There is no known effect on proliferative ability or biology of cells and they are well retained in cells for several days post-stain. Available kits for flow cytometry include CellTrace CFSE Cell Proliferation Kit (Cat. No. C34554), CellTrace Violet Cell Proliferation Kit (Cat. No. C34557...
emission of CellTrace™ Violet dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry) (see Fluorescence Spectra for CellTrace™ Violet stain ...
CellTrace™ Violet Cell Proliferation Kit Catalog no. C34557 Introduction The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-use form. The kit contains CellTrace™ Violet in nine single-use vials to permit ...
品名:CellTrace™ Violet Cell Proliferation Kit, for flow cytometry 规格:180 assays 储存条件: Contains 9 vials of CellTrace™ Violet (lyophilized powder) and 1 vial of DMSO (500 µL). Store in freezer (-5 to -30°C) and protect from light. ...
To determine target cell lysis through flow cytometry-based assays, tumor cells were loaded with 2.5 µM CellTrace™ Violet (Thermo Fisher Scientific) and cultured with PBMC (E:T 5:1) in the presence or absence of CC-3 or isotype control (1 nM each). Standard calibration beads (Sigma...
(a fluorescent dye that is diluted with cell proliferation) were cocultured with ID8 or ID8.hMeso target cells in the presence of flow-sorted FRβ−or FRβ+TAMs for 72 h.bRepresentative histograms looking at cell trace violet staining on CAR+T cells (gated on live, CD3+GFP+, left...
To evaluate B cell clearance potential of CAR T cells, CTV dilution assay was used. 5 × 106CAR T cells were labeled with 1 µM CellTrace Violet (CTV) dye (Thermo Fisher Scientific) according to the manufacturer’s instructions. Labeled cells were co-cultured with 2.5 × 106CD...
CellTrace™ CFSE 细胞增殖试剂盒适用于体外和体内标记细胞,采用染料稀释法通过流式细胞分析追踪多代细胞。•卓越的性能 — 明亮的单峰染色可实现多代细胞可视化•信号稳定时间长 — 染色后可在细胞中保留数天•无毒 — 染色不会对细胞健康产生不利影响•简...
The CellTrace Violet Cell Proliferation Kit (C34557) includes the CellTrace Violet stain together with dimethylsulfoxide (DMSO) for preparation of a stock solution. Figure 15.4.7 Human peripheral blood lymphocytes were harvested ...