CellTrace Violet (CTV) is a powerful tool for tracking cell proliferation by permanently binding cellular proteins and rendering the cell fluorescent. After cell division, each daughter cell contains half of the parent cell's fluorescence, enabling quantification of proliferation via flow cytometry. ...
用CellTrace Violet(CTV)标记4-羟基-3-硝基苯基乙酰基(NP)特异性B1-8hi AicdaGFP/+B细胞,并将其过继转移到小鼠体内。由于敲除B1-8hi重链与Igl配对产生NP的高亲和力受体,他们在免疫后7天检测了Igλ+B1-8hi CD45.1+细胞的表型。GL7在第一轮细胞分裂中上调。原始标记物CD38和IgD的丢失以及相应的GC标记物CD95...
m, n, Myeloid suppression assay representative histogram of CD8A+ OT-I T cell dilution of CellTrace Violet (CTV), indicative of proliferation (m), and quantification of division index (n) for MC38 tumour myeloid cells isolated using CD11B+ microbeads from rapamycin- and vehicle-treated mice ...
d, e, Ex vivo suppression of CellTrace Violet (CTV)-labeled WT naïve CD8+ T cell proliferation by Jmjd1cTreg WT and Jmjd1cTreg KO Treg cells sorted from tumors (d) or spleens (e) with the annotated co-culture ratios. Teff, CD8+ T effector cells. n = 3 biological independent ...
(G–L) 19-28z CAR-T cells expressing LNGFR or GLUT1 were pre-labelled with cell trace violet (CTV) and stimulated with NALM6 under 10 mM, 5 mM, 1 mM, and 0.1 mM glucose concentrations. Five days later, CTV distribution was measured by flow cytometry. CTV levels are quantified from ...
CellTrace Violet (CTV)-labeled splenocytes from LCMV-infected mice were treated with brefeldin A for 12 h to block IFN-γ secretion beginning at 72, 96, and 120 h post-peptide stimulation. A statistically significant increase in the frequency and total number of proliferating IFN-γ+CD8+...
(Fig. 2h). Here, the increase in CellTrace Violet (CTV) dilution signifies enhanced proliferative capacity of the Ptpn2-deleted Slamf6+ cells compared to control cells. We also compared the proliferative capacity of antigen-experienced control and Ptpn2-deleted Tim-3+ cells following re...
Sorted naïve T cells were labelled with CellTrace Violet dye (C34557, ThermoFisher Scientific) and plated onto a 96-well round-bottom plate pre-coated with 1 µg/ml purified NA/LE hamster anti-mouse CD3 (Table S2). Myeloid cells and T cells were plated at 1:5 ratio (2 × ...
(h) Representative flow cytometry plots of Cell Trace Violet (CTV) following 72 hours of in vitro stimulation (left), and quantification of division number by CTV dilution (right) in EnhDel and WT cells. Dots represent individual mice; n = 8 mice for EnhDel, n = 6 mice for...
(Fig.1c). The mice were also given transfers of CellTrace Violet (CTV)-labeled follicular B cells 1 or 2 d before to help identify lymphoid follicles. To guide expectations for the intravital imaging analysis, which can detect fluorescent cells at depths of up to 200 µm, thin ...