Cell viability assay After puromycin selection, cells expressing controls and candidates’ guides were collected and seeded in 96-well plates in at least three technical replicates for each timepoint (3000 cells per well). Proliferation assay was performed using the Cell-Titer Glo 2.0 (Promega) rea...
G 3D spheroids made of either Meso 8 cells alone or cocultures of Meso 8 and AF 8 were treated with cisplatin/pemetrexed or diluent (NT). Left panel: representative images of n = 3. Scale bar: 1 mm. Right panel: Cell viability was determined using the Cell TiterGlo 3D assay....
drug test and regeneration medicine. Organoids derived from adult stem cells possess the capability to maintain self-renewal while being able to differentiate into functional cell types, mimic the three-dimension (3D) structures and retain the functions of the origin tissues (Schutgens and ...
period using the Cell Titer-Glo assay. By comparison with WT hESCs, H3K27M hESC spheres exhibited increased cell number (Fig. 1B and Additional file 2: Table S1) and concordantly, were also of a larger size (Fig. 1C). In addition, we observed that H3K27M cells formed a greater ...
For clinical trials that required high vector administration, a sensitive assay to detect Nab may not be necessarily required.32 Cumulatively, the therapeutic effect of systemic gene therapy in clinical practice is determined according to the AAV vector dose, serotype’s propensity, and Nab titer in...
verticality like we do.” By incorporating quick startup, automation, software wizards, and easy training, added Rogers, the Bigfoot Spectral Cell Sorter has proved that a high-end sorter can indeed be operated successfully by end users, freeing staff to focus on new ...
Cell Titer Turbo 2.0 Luminescent Cell Viability Assay 借助ATP 依赖的荧光素酶催化的荧光素发光反应,通过化学发光信号测定细胞内ATP 含量,从而检测细胞活力或定量检测活细胞数目,检测线性范围宽、灵敏度高、稳定性好。96 孔板中,在100 个至100,000 个细胞范围内有良好的线性关系,但不同细胞的检测数量上限会有不同...
To assess the viability of OA treated HLO, 3D cell titer glo assay was performed (Promega, Wi, USA). These organoids were further assayed by live-cell imaging, qRT-PCR, and stiffness analysis. Lipids and Membrane Live-Cell Imaging After being cultured in an ultra-low attachment 6 multi-...
Proliferation assay A total of 1 × 104 BMDM were seeded in a 96-well plate and cultured for 24 h in DMEM with or without 3 nM recombinant RARRES2 (R&D, 2325-CM-025). The cells were lysed using cell titer Glo (Promega, G7572) according to the manufacturer’s instructions....
The selectivity of ATP-Red 1 was independently confirmed, by using Cell-Titer-Glo to measure ATP levels in cells, after flow cytometry. However, as Cell-Titer-Glo is a luciferase-based assay, it requires cell lysis to detect intracellular ATP levels and cannot be used for live-cell sorting...