网络释义 1. 细胞悬浮液 批发采购生化... ... Cell Wash Buffer( 细胞清洗液)Cell Suspension Solution(细胞悬浮液) Cell Lysis Solution( 细胞裂解 … detail.china.alibaba.com|基于9个网页
One milliliter of 10% Optiprep (cat.n. D1556, Sigma Aldrich) in NMDG-CS was loaded under the cell suspension solution using a gel loading tip and centrifugated at 200g for 6 min with a low break. Supernatant was discarded, the cell pellet re-suspended in NMDG-CS + B27 ...
Cell suspension was applied to the column and all solution was allowed to flow through. Columns were further washed one with 500 μl MACS buffer. The eluent containing enriched T cells were collected and centrifuged at 300 g for 5 min at 4°C. Pellet was resuspended in 1 ml of...
Cells were lifted for evaluation by incubating them with CTS EDTA Versene Solution for 8 min at 37 °C. The 3D suspension expansion protocol was modified from previously published reports by Borys et al. [27], Rohani et al. [32], and Dang et al. [28]. For expansion, 2 × 106 ...
The single-cell suspension from explants were harvested at 0, 1, 6, and 12 HACI with two replicates. In brief, the hypocotyl was chopped into small discs with scalpel blade and immersed in 3–5 ml RNase-free enzyme solution (1.5% cellulase R10, 1.5% macerozyme R10, 0.4M mannitol, 10mM...
Suspension components are separated using centrifugation based on their sizes, shapes, densities, centrifugal vigor, and solvent stickiness. Significant centrifugal forces at up to 1,000,000g were utilized in ultracentrifugation to separate MSC-Exos from various sample components [43]. The tangential ...
Following suitable cell growth, for example, after a two-week cell culturing period at 35° C to 37° C, the cells can be harvested from the suspension such as be sedimentation or centrifugation at 200-1000 g. The packed cells are then thoroughly washed such as in saline solution, lactated...
Importantly, we do not apply any cell sorting and attempt to randomly sample the cell suspension. Additional file 1: Table S1 and Figure S1B summarizes the data in terms of patient diagnosis and cell numbers, molecular, and gene detection rates, which are comparable to those obtained in ...
Microglia are resident myeloid cells in the central nervous system (CNS) that control homeostasis and protect CNS from damage and infections. Microglia and peripheral myeloid cells accumulate and adapt tumor supporting roles in human glioblastomas that s
24 September 2020 Main Single-cell and single-nucleus RNA sequencing (scRNA-seq and snRNA-seq, respectively) represent powerful new tools to identify cell types and their respective transcriptional signatures that reside within healthy and diseased tissues. Before the development of these technologies, ...