Humphries MJ. Cell-substrate adhesion assays. Curr. Protoc. Cell Biol. 2001 Chapter 9, Unit 9.1.Humphries MJ. Cell-Substrate Adhesion Assays. In: Bonifacino JS et al., eds. Current Protocols in Cell Biology. New York John Wiley and Sons, Inc., 2003:9.1.1-9.1.11....
Intracellular motility and cell substrate adhesion has been investigated using magnetic field relaxation of ingested magnetic particles and interference reflection microscopy (IRM). Alveolar macrophages were used in the model cell system. Cell motility for non-stimulated cells was found to be essentially ...
In the first protocol presented in this unit, physical adhesion of a cell is assessed by determining the extent to which the cell spreads on a defined substrate--the plate is coated with the test substance, cells are added and allowed time to attach and spread, and the extent of spreading...
A similar, but less marked, increase in c-Src PTK activity occurs upon incubation of CEFs in calcium-free phosphate-buffered saline, which also causes a decrease in cell–substrate adhesion. The increase in c-Src PTK activity following disruption of cell–substrate adhesion correlates with a ...
Eps15 (epidermal growth actor receptor pathway substrate 15), sca olding and clustering protein with multiple clathrin and AP-2 binding sites and three Eps15- homology (EH) domains Epsin (Eps15-interacting protein), adaptor or ubiquitinated cargo and membrane bending protein that associates with ...
The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE Lasse Wolfram ,Clara Gimpel &Günther Schlunck Article 21 March 2024|Open Access Mechanosensitive TRPV4 channel guides maturation and organization of the bilayered mammary epithelium ...
Xcad-11 localization at the cell–substrate interface pointed towards a functional role of Xcad-11 in FA formation and/or cell–substrate adhesion. To test this hypothesis, Xenopus embryos were injected with control or Xcad-11 antisense MO. NCC were then explanted on fibronectin-coated dishes, fi...
we found that adhesion between border cells and their substrate, the nurse cells, functions in a positive feedback loop with Rac and actin assembly to stabilize forward-directed protrusion and directionally persistent movement. Adhesion between individual border cells communicates direction from the lead...
[193]. It is responsible for the drug resistance in most of lung cancerous cells [194]. Another tumor marker that mediates critical processes for cancer progression, such as migration, cell adhesion, and tumorigenesis is osteopontin. Its expression rate in tumor cells is 67.8%, while only ...
Nonenzymatic lysined-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses Qihang Zhao Qiang Wang Pin Wang ArticleOpen Access06 Jan 2025 Donor MHC-specific thymus vaccination allows for immunocompatible allotransplantation ...