e Cell type enrichment for the POAG and IOP GWAS was corroborated using two regression-based methods that assess cell type-specificity of trait associations considering all associations genome-wide: stratified-LD score regression (S-LDSC) and MAGMA. Full size image POAG and IOP associations ...
Full size image CX43 overexpression on BMSCs maintained KG-1a cells stemness in vitro and vivo KG-1a cells, which were isolated after co-culturing with CX43-BMSCs, met more S and G1 phases than the ones that co-cultured with EV-BMSCs or from under VCR function with CX43-BMSCs (Fig...
Scale bars, 5 µm. Scanning electron microscopy To examine the effects of the two actin-binding agents on LSEC fenestration, purified cells were grown on collagen- coated cover slips and prepared for SEM. In these experi- ments, we treated cells with 100 nM of one of the com- pounds, ...
Download: Download full-size image Figure 1. Single-cell transcriptome profiling and analysis of the human fetal and postnatal testis (A) Dimension reduction presentation (via UMAP) of combined single-cell transcriptome data from embryonic, fetal, and infant human testes (n = 30,045). Each dot...
Scale bar: 100 μm. Full size image Drop-BS allows processing a large number of cells or nuclei. Our scDNA droplet generation was conducted with rate of ~3000 droplets per second while barcode droplet generation and fusion with scDNA droplets at the rate of ~132 droplets per second. In...
6903. Utah State University; 2017. https://digitalcommons.usu.edu/etd/6903 Jakočiūnas T, Klitgaard AK, Kontou EE, Nielsen JB, Thomsen E, Romero-Suarez D, et al. Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast. Synth Syst Biotechnol 2020;5:11–8...
The three single-cell datasets used in this study underwent initial quality control by the original authors, and subsequent independent analyses were performed on each dataset. The expression of all cells was normalized using the “LogNormalize” function with a scale factor of 10,000. The top 20...
Scale bar, 10 μm. (C) Cell counts performed at 24 and 48 hr post plating equal numbers of shRNA-infected cells (24 hr post infection). Cell number is normalized to 0 hr. (D) EdU and propidium iodide (PI) flow cytometry analysis of cell cycle in Rh30 cells at 4 days post ...
In the 'no diabetes' group, sample size/number of studies were equal to 118,448/45, 36,433/13 and 154,881/58, in the discovery, replication and combined analyses, respectively. In the 'overall' group, the numbers for the three analyses were equal to 133,413/48, 42,116/14 and 175...
Recent data have shown that mouse and primate mitotic spindle size asymmetry is a key component of asymmetric cell division in the cerebral cortex13 independently of spindle orientation14. Despite that, abnormal cleavage orientation remains the exclusive explanation for the declines in symmetric ...