GAPDH (D16H11) XP®Rabbit mAb detects endogenous levels of total GAPDH protein. Species Reactivity: Human, Mouse, Rat, Monkey Species predicted to react based on 100% sequence homology: Pig Source / Purification Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresp...
B Cell Signaling Antibody Sampler Kit Confocal immunofluorescent analysis of Ramos cells, serum-starved (overnight; left) or IgM-treated (12 ug/ml, 2 minutes; right), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye)....
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CST 8079S IFI16 Antibody 100µl ￥3,100 NF-kB Signaling Polyclonal Antibody -20C CST 8080S TFIIF-α Antibody 100µl ￥3,100 Chromatin Regulation / Epigenetics Polyclonal Antibody -20C CST 8081S K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb 100µl ￥3,900 Protein Stability Mono...
GAPDH, glyceraldehyde 3-phosphate dehydrogenase. h, ESCs synchronized with double thymidine block, existing 2CLCs removed by FACS and IAA added as indicated. Emerging 2CLCs were quantified 6 h after release. Statistical analyses for pairwise comparison with control group were with a two-sided ...
(Cell Signaling Technology, #9027, 1:400) were incubated overnight at 4 °C. Staining was performed using a VECTASTAIN Elite ABC kit (Vector Laboratories, #PK-6101) and DAB peroxidase substrate kit (Vector Laboratories, #SK-4100). Immunostaining was analyzed using the immunoreactive score59....
GAPDH was used as an internal control. i The treatment with 5 μM of 5-aza-2-deoxy cytidine reduced the DNA methylation level of multiple CpG sites throughout the ZNF471 promoter in SiHa and CaSki cells Full size image ZNF471 expression is silenced due to promoter methylation ZNF471gene ...
The dysfunction of GAPDH and PGDH leads to the depletion of post-GAPDH glycolytic intermediates and the 3-phosphoglycerate derivative serine that are important fuels for T cell proliferation. The environmental lactate eventually suppresses effector T cell proliferation. Full size image Overall, as ...
mRNA expression values were calculated using the ΔΔCt method and GAPDH gene as a control. A detailed list with primers used in the present study is provided in Table S7. CT values were shown in Supplementary Material 1. Western blotting Total cell lysates were prepared in RIPA lysis buffer...