The number of cells in alginate droplets was linearly proportional to the cell seeding density. The doubling time of A549 and PC9 cells were 15.9 h and 16.1 h, respectively, which were similar to those obtained from traditional 2D cell cultures. IC 50 values from A549 and PC9 cells exposed ...
96-well Collection Plate 96-well receiver trays with lids for Millicell®-96 Cell Culture Insert Plates, sterile, pack of 5; Synonyms: 96-well collection plate,Collection plate,Microplate at Sigma-Aldrich
as is the case in the above showing the evaluation of cardiomyocyte mechanisms of action. H9c2 cells were seeded at 2,000 cells per well in 96-well plates. Two hours after seeding, the cells were treated with the listed compounds for 4, 24, or 96 hours before immunofluorescent labeling an...
A custom-made esiRNA library was purchased from Sigma/Eupheria Biotech (MISSION® esiRNA; Supplementary Table6). Cells were seeded into 96-well plates (flat-bottom, Costar) at a density of 7000 cells/well. Twenty-four hour after seeding, esiRNA was transfected in triplicates at a concentrati...
Extreme limiting dilution assay was performed as described [63,64]. Different dilutions (1, 10, 25, 50 cells/well) for HCT 116 cells were seeded in 96-well plates and treated with BSA or BSA-PA/OA (PA 100 µM, OA 200 µM) respectively. After 2 weeks, the number of sphere...
Compared to CellTiter-Glo, the MicroColonyChip is as sensitive and also robust to artifacts caused by differences in initial cell seeding density. We demonstrate efficacy via studies of radiosensitivity and chemosensitivity and show that the approach is amenable to multiplexing. We conclude that the ...
Cell culture media tend to foam due to the high protein content of supplemented serum. Air bubbles can hinder cell attachment during seeding and therefore create well-to-well variations of cell numbers (Image 2). Especially in smaller plate formats like...
*Seeding density is given for each culture vessel type as follows: Dishes and Flasks: Cells per vessel; Culture plates: Cells per well. †The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used....
Cultures were passaged in M1 using three individuals per species, in triplicate, in 24-well plates (Falcon). All three species were cultured at both ~22 °C and 4 °C. The seeding density was 3.00E + 06 cells/mL forG. barretti, and 5.00E + 06 cells/mL forGeodiasp...
NSCs cultured on ONAS proliferated better than those on the flat surface when cell density was low and showed less spontaneous differentiation during proliferation in the presence of mitogens. Interestingly, NSCs cultured on ONAS at clonal density demonstrated a propensity to generate neurospheres, ...