mRNA quality was assessed by measuring the optical density (OD) at 260/280 nm (nucleic acids/protein ratio), 260/230 nm (nucleic acids/guadinine salts), nucleic acids concentration (Nanophotometer, Implen, Germany) and the RNA integrity number (RIN value, 2100 Bioanalyzer System, ...
At osteogenic differentiation, there was a deposition of calcium salts in an extracellular matrix using Alizarin Red S (“Sigma–Aldrich”, St. Louis, MO, USA) and positive staining for alkaline phosphatase using 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT) (“Sigma–...