Ciliated cells elevated the expression of interferon-stimulated genes (Fig.3e, fand Supplementary Data5), which are involved in viral response mechanisms and are crucial in driving and maintaining lung inflammation, for example, by promoting antigen presentation or stimulating cytokine production43. Cili...
differentiation. Experimentally, evidence has shown that the transplantation of allogenic insulin-producing cells derived from MSCs is associated with a muted allogeneic response that does not interfere with their functionality. This can be explained by the immunomodulatory functions of the MSC subpopulation...
Although comprehensive cell-surface protein maps are highly valuable, they have been difficult to define until recently. In this review, we discuss the application of a contemporary targeted chemoproteomic-based technique for defining the cell-surface proteomes of stem and progenitor cells. In applying...
Interestingly, a recent dose-escalation study documented that intravenous infusions of AT-MSCs from healthy donors are well tolerated in humans up to 4 × 106 cells/kg body weight, when a significant increase in clotting parameters and fibrinolytic response became apparent [25]. Importantly, AT-...
Application of MSCs as cell therapy is based on regulating the inflammatory response and participating in tissue repair and regeneration [17]. The therapeutic effect of MSCs is mainly attributed to their immunomodulatory function regulated by the inflammatory environment [18]. When stimulated by inflamma...
Multiple clones of CSCs pre-exist, and some can adopt and expand easily to changes in the tumor microenvironment (TME) and/or in response to radio- and chemotherapy. A combination of both intrinsic and extrinsic factors contributes to CSC-mediated therapy resistance. In this review, we will ...
Hyperactive coagulation might cause dangerous complications such as portal vein thrombosis and pulmonary embolism after mesenchymal stem/stromal cell (MSC) therapy. Tissue factor (TF), an initiator of the extrinsic coagulation pathway, has been suggested
directions. This concise review summarises recent findings and highlights new directions for the application of high-throughput single-cell and spatial technologies with multiomics to unravel previously unknown mechanisms for the advancement of new therapeutics targeting liver pathology in response to ...
Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved tissue is essential to move this emerging immune monitoring technology from the bench to the bedside. Improper sample preparation leads to detrimental cascades,
Cellular stages of biological processes have been characterized using fluorescence-activated cell sorting and genetic perturbations, charting a limited landscape of cellular states. Time series transcriptome data can help define new cellular states at th