BD™ Cell Recovery Solution is used full strength to recover cells from BD Matrigel® Matrix. Complete protocol provided with product. Formulation Nonenzymatic proprietary solution product details at-a-glance Qty./Pk.:1 Qty./CS.:1 Size:100 mL ...
HandlingCorning Cell Recovery Solution is used full strength to recover cells from CorningMatrigel® Matrix. Complete protocol provided with product. FormulationNonenzymaticproprietary solution 产品描述 Product Details At-a-Glance Qty./Pk.: 1
(Fig.1c, d), indicating that the majority of metastatic foci in the liver in this model originate from cell clusters. We further examined the liver tissues at seven weeks after transplantation using a tissue-clearing protocol called a clear, unobstructed brain/body imaging cocktails and ...
Transfection of Expi293F cells was conducted using the Expi293™ Expression System Kit (A146315; Thermo Fisher Scientific) following the manufacturer’s protocol. The protein was purified from the cell culture supernatant using recombinant Protein A affinity chromatography (HiTram MabSelect SuRe, ...
The cell recovery protocol was performed with fixation within 4 h of HIFU exposure for all lesions. Fixation was performed within this short post-recovery period to avoid ensuing cell death due to removal of the liver tissue from its blood supply. The following day, resulting cell suspensions ...
Single-cell suspensions were loaded onto a Chromium Single Cell Controller (10x Genomics) to generate Gel Bead-In-Emulsions (GEMs). cDNA libraries were prepared using the single cell 5' solution v2 reagent kit (Chromium, 1000020) according to the protocol provided in the 10x Genomics Chromium Si...
For RNA isolation from small intestinal organoid cultures, purified IEC were recovered from Matrigel using the BD Cell Recovery Solution following the manufacturer’s instructions and cell lysates were prepared with RLT buffer. RNA extraction was performed with the RNeasy Mini Kit (Qiagen), followed ...
organoids were removed from the 6-well culture plates using medium and P1000 pipettes and spun down for 8 min at 500 gwith the brakes off. Cell pellets were then incubated with Cell Recovery Solution (Roche Diagnostics) for 30 min on ice to remove excess BME. Cells liberated from ...
The protocol for cross-linking and purification of protein-DNA complexes was described previously5,71. In brief, cells (1 × 107) were fixed by incubation for 10 min at 37 °C with 5 ml of 1% formaldehyde in PBS, after which glycine was added to a final concentration of 0.25...
Protocol economics To compare costs per sample across the tested methods, we iteratively downsampled the fragments files for each sample and calculated the fraction of duplicate fragments per iteration. We then calculated the average sequencing depth at which a duplication rate of 50% was attained an...