Quality and quantity of DNA were assessed using Nano-Drop ND-1000 Spectrophotometer (Nano Drop Technologies, Wilmington, DE), agarose gel electrophoresis and quantitative polymerase chain reactions (qPCR). Genes of interest Based on a deep literature review, 25 candidate genes are known to be ...
(Kapa Biosystems, Wilmington, MA, USA, Cat KR1351) according to the manufacturer’s protocol. All library samples were validated with the Agilent Bioanalyzer, quantified with Qubit, and sequenced on Illumina HiSeq 2500 (San Diego, CA, USA) with single-read mode/50 million depths. For ...