In modern biology, the correct identification of cell types is required for the developmental study of tissues and organs and the production of functional cells for cell therapies and disease modeling. For decades, cell types have been defined on the basis of morphological and physiological markers ...
Subpopulations of tumor cells exhibited signatures of proliferation, KRAS signaling, cell stress and epithelial-to-mesenchymal transition. Mapping mutations and copy number events distinguished tumor populations from normal and transitional cells, including acinar-to-ductal metaplasia and pancreatic ...
Our toolkit also includes a set of refined functional assays to verify microglial identity. We demonstrate the effectiveness of the Tet-regulated CRISPR-dCas9 system to modify gene expression in iMG, bypassing DNA double-strand breaks through CRISPR-interference (CRISPRi) and CRISPR activation (CRISPR...
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Immunofluorescences and fluorescence in situ hybridization were performed to verify cell identity. Results In this study, we constructed a single-cell landscape covering 88,013 high-quality (500 < genes < 4,000, UMI < 50, 000, and mitochondrial gene ratio < 40% or 15%) single cells and ...
(cell number, cells or nuclei, sequencing depth, etc.). In order to accurately determine the efficiency of demultiplexing, we hashed different cell lines that carry intrinsic genetic variations, which serve as a ground truth control. Importantly, for one of the hashing datasets, we compared two...
When I open my workbook, I get a message that it contains links to one or more external sources. It shouldn't. Recently had this and a couple of...
reason reason string Text reason for the failure (if not successful) reasonSupport reasonSupport string Additional information about the failure (if not successful) response response array of OrganizationSummary Response payload returned returned integer Number of organizations returned by search,...
Fig. 1: CIGdiscover uncovers cell identity genes (CIGs) on the basis of the unique histone codes for their transcriptional regulation. a, b The number of curated CIGs for each category (a) or cell type (b). c Heatmap to show −log10 P-value for difference in histone modification feat...
2i). This suggested that in vitro culture does not change the identity of the DDX4 Ab+ cells. We next wanted to verify the localization of DDX4 Ab signal to perivascular cells in the human ovarian cortex. We chose two highly expressed markers from the perivascular cluster, MCAM and RGS5...