Thescratch assay is an easy, low-cost and well-developed method to measure cell migration. The basic steps involve creating a "scratch" in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to ...
BioTek AutoScratch wound making tool automatically creates reproducible scratch wounds in cell monolayers. BioTek MultiFlo FX dispenser automates microplate washing.
Liang CC, Park AY, Guan JLIn vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2(2): 329-333 The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro. The basic steps involve...
In vitro wound-healing assay (scratch assay) This method is a non-demanding and cheap assay to study the migration of cells on 2Dsurfaces and mimics cell migration during wound healingin vivo. In this assay, when the cells grow to a single layer state, a blank area is created in the mo...
Cell migration through the extracellular matrix (ECM) is one of the key features for physiological and pathological processes such as angiogenesis, cancer ... GS Jeong,HK Gu,AR Kang,... - 《Biomedical Microdevices》 被引量: 30发表: 2011年 Cell migration analysis: Segmenting scratch assay images...
We also evaluated the influence of the combined treatment with melatonin and vemurafenib on the cell migration and invasion ability in melanoma cells. The scratch assay was employed to determine the combined effect of vemurafenib with melatonin on cell migration in melanoma cells. We found that the...
Liang, C., Park A. Y., and Guan J. (2007)In vitroscratch assay: a convenient and inexpensive method for analysis of cell migrationin vitro.Nat. Prot.2, 329–333. Vial, E., Sahai, E., Marshall, C. J. (2003) ERK-MAPK signalling co-ordinately regulates activity of Rac1 and RhoA...
Cell migration, a fundamental process in development, wound healing, and immune function, is a common topic in undergraduate cell biology courses. We developed laboratory exercises with an inquiry-based learning (IBL) approach in which cell migration could be examined with the scratch assay, adapted...
the in vitro scratch assay, the cell distribution images were acquired at 0 and 24 h after stimulation, and the scratch areas were quantified by Image J 1.52 software. The initial area was marked as Siand the residual area was marked as Sr. The rate of migration was calculated as ...
migration assays showed that depletion of NudCL2 increased single-cell migration (Fig.1b, c). Tracing the migratory path of live cells by time-lapse microscopy revealed that knockdown of NudCL2 increased the speed of single-cell motility (Fig.1d–f). Interestingly, wound healing assay showed ...