According to the fluid mosaic model, the cell membrane is a solution of integral proteins with two-dimensional orientation in a viscous phospholipid bilayer solvent that is considered fairly structureless. [9] This model is, however, a highly simplified model of the cell membrane, as the lipid ...
Membrane patch size = 19 Minimum sigma = 1.0 Maximum sigma = 16.0 We additionally use a Bayesian Network model, which is also implemented in Weka4. This approach, called BayesNet, follows a Bayesian statistical model to determine the probability that observed features are conditionally dependen...
2d). Similarly, sGRAPHIC efficiently labeled neighboring cells with GFP in co-culture of HeLa cells stably expressing sGRAPHIC reporters (Supplementary Fig. 2e). Reconstituted GFPs were predominantly localized to the cell membrane as demonstrated by the loss of GFP signals after promiscuous digestive ...
We asked whether the structure and function of a broad neutralizing antibody (bNAb) epitope, the membrane-proximal ectodomain region (MPER) of the viral gp41 transmembrane glycoprotein, differ in cell-free and cell–cell-transmitted viruses and whether this difference could be related to Ab ...
Mask expansion extends the boundary of each segmented nucleus by a user-specified number of pixels to capture cell membrane fluorescent signal (Fig. 1, step 4). Lateral bleed compensation aims to correct fluorescent signal spillover between adjacent cells, an issue often seen in immunofluorescence ...
First linked to cancer through the finding that resting membrane potential in neuroblastoma cells was linked to proliferation, Kv11.1 has since been found to be expressed at high levels in multiple tumor types [41]. While this potentially makes Kv11.1 a good target, it is also expressed in ...
the two sides of a scratch created by a 200-μL pipet tip after 24 h (Fig.4c). Moreover, as illustrated in Fig.4d, the two-chamber migration assay and crystal violet staining showed that melatonin treatment for 24 h increased the number of cells on the bottom of the membrane ...
When the cation-exchange membrane was cracked or the electric current dropped independent of pH, the biofilm-bearing anode electrodes were taken out from the anode chamber and transferred to a new anode chamber containing the fresh medium; this was done in an anaerobic glove box filled with N2 ...
Separated proteins were transferred to nitrocellulose membrane and blocked with blocking buffer (5% non-fat milk in TBST (20 mM Tris–HCl, pH 7.4, 110 mM NaCl, 5 mM MgCl2, 0.1% Tween 20). Primary antibodies (Table S13) were prepared in the blocking solution and membranes were incubated...
Red: Rhodamine-phalloidin-labeled-cell membrane; Cyan: P-F8-DPSB-labeled NPs. i Live/dead analysis of LN-TCV before inactivation (before) and cultured (day 1, 2, and 4) after inactivation for demonstrating inactivated LN-TCV. Green: live cells; Red: dead cells. The images were presented...