The resulting hybrid clones express one or more T-cell-specific surface components identifiable by radioiodination of intact cells followed by NP-40 solubilization of membrane proteins and sodium dodecyl sulfate polyacrylamide gel electrophoresis. These markers are stably expressed by the hybrid lines ...
However, there should be no long-term changes in pH in the lungs after inhalation of NaHCO3, as any elevation in pH should be rapidly neutralized by Na+ influx into the cytoplasm of AECs via ENaC and across the basolateral membrane into bodily fluids. Otherwise, the increased Na+ ...
The labeled exosome suspensions were filtered using a 100-kDa MWCO hollow fiber membrane (Thermo Fisher Scientific) to remove the excess dye. HUVECs were seeded in 6-well plates and incubated with the Dil-labeled exosomes (100 μg/mL) for 24 h. Then the cells were fixed with 4% ...
Tikhonov A. N. (2018). The cytochromeb6fcomplex: biophysical aspects of its functioning in chloroplasts. In J. R. Harris & E. J. Boekema (Eds.),Membrane protein complexes: structure and function, subcellular biochemistry. Vol. 87 (pp. 287–328). New York: Springer Nature Singapore Pte L...
1a). The secreted EVs were isolated from hCVPCs and showed a double-membrane-bound, cup-shaped typical shape (Fig. 1b). Nanoparticle tracking analysis (NTA) confirmed the mode size of secreted EVs from hCVPCs was around 118 nm in the EV-N and 110 nm in the EV-H (Fig. 1c),...
The latent membrane protein-1 (LMP1) of EBV, may play a causal role in the development of this disease. The experiments initiated here were designed to examine the activity of LMP1 in vivo in the epidermis of PyLMP1 transgenic mice in relation to its putative role in carcinogenesis. ...
Dissection of the Dislocation Pathway for Type I Membrane Proteins with a New Small Molecule Inhibitor, Eeyarestatin The mammalian endoplasmic reticulum (ER)-to-cytosol degradation pathway for disposal of misfolded proteins is an attractive target for therapeutic interven... Fiebiger,E. - 《Molecular...
The design of CAR-T cell therapy and a simplified basis for classifying TIME.ACAR-T cell therapy’s design and process. The process of CAR-T cell therapy mainly includes four steps: firstly, T cells are extracted from peripheral blood, and then CAR-T cells are created by modification. Thes...
Analyzing proteins from single cells by tandem mass spectrometry (MS) has recently become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and reproduc
removing the soybeans to give the immersion water as an extracted solution with water, removing protein from the extracted solution by an ultrafilter membrane to give a filtrate, bringing the filtrate into contact with an adsorbent and eluting the glycoside from the adsorbed material by using an ...