Cell lysis buffers for mammalian tissues T-PER Tissue Protein Extraction Reagent N-PER Neuronal Protein Extraction Reagent When to use (compatible sample types) Heart, liver, kidney, lung, spleen Brain tissue and primary neurons Composition Non-de...
Composition 25 mM Tris-HCl, pH 7.6 150 mM NaCl 1% NP-40 1% sodium deoxycholate 0.1% SDS A modified RIPA buffer formulation without SDS Non-denaturing detergent in 25 mM bicine buffer (pH 7.6) 50 mM Tris, pH 7.4 250 mM NaCl 5 mM EDTA...
Cell Lysis Buffer (10X)Description: Cell Lysis Buffer is used to lyse cells under nondenaturing conditions.1X Cell Lysis Buffer: 20 mM Tris-HCl (pH 7.5) 150 mM NaCl 1 mM Na2EDTA 1 mM EGTA 1% Triton 2.5 mM sodium pyrophosphate 1 mM b -glycerophosphate 1 mM Na 3VO 4 1 µg/...
The measured stiffness of fibrin hydrogels, with the composition tested in the current study, was in the range of the stiffness of the native pulp tissue, which has previously been reported to be 800 Pa38. In addition, the stiffness of SAP hydrogels was found to be sevenfold higher than the...
To lyse cells, isolated cells were added to a 0.2 ml thin-wall PCR tube containing 2 μl of a mild hypotonic lysis buffer composed of 0.2% Triton X-100 (Sigma–Aldrich, Germany) and 2U/μl of recombinant RNase inhibitor (40 U/μl, Takara, Japan), 1 μl of 10 mM oligo...
Our results demonstrate a dynamic change in cell composition in the niches of each type AnLT during both antler generation and regeneration; further, the tissue-specific characteristics of each AnLTs may be reflected by their specific cell composition (as compared with the non-antlerogenic tissue)...
Cell composition deconvolution was conducted utilizing CIBERSORTx [24]. Our initial step was to generate a signature gene expression matrix using the CRC scRNA-seq dataset (GSE178341). We extracted raw count matrix data and cell type classifications from a subset of the Seurat object, which incorp...
An extensive intravacuolar network forms inside the parasitophorous vacuole (PV). This is a loose meshwork of tubules of unknown composition and function. The IPN has been reported to be continuous with the PVM suggesting it may be used to increase the effectivesurface areaof the PVM, although...
Serum-starved cells are treated as desired, washed with ice-cold phosphate-buffered saline [PBS, 10 mMNa2HPO4-HCl (pH 7.3), 1.5 mMKH2PO4, 3 mMKCl, 137 mMNaCl], and lysed in ice-cold NP-40 lysis buffer (400μl for a 10-cm dish). All subsequent steps are performed at 4°. Lysa...
Mammalian tissue cell lysis buffers T-PER Tissue Protein Extraction ReagentN-PER Neuronal Protein Extraction Reagent Compatible sample typesHeart, liver, kidney, lung, spleenBrain tissue and primary neurons CompositionNon-denaturing detergent...