误区七:STR鉴定只需检测规定的核心位点即可根据2011版《Authentication of Human Cell Lines: Standardization of STR Profiling》(ANSI/ATCC ASN-0002-2011)的建议,进行细胞STR鉴定时可比对的核心位点有8个常染色体位点(CSF1PO、D5S818、D7S820、D13S317、D16S539、TH01、TPOX、vWA)以及Amelogenin位点。新版的...
ATCC的标准发展组织 (Standards Development Organization, SDO) 颁布了ASN-0002标准《Authentication of Human Cell Lines: Standardization of STR Profiling》。4.采用STR分型技术鉴定细胞株时,需要比对多少个位点才能确认细胞身份?答:根据ANSI/ATCC ASN-0002标准,采用STR分型对细胞株进行鉴定时,比对8个核心位点以...
美国国立卫生研究院 (National Institutes of Health, NIH)、国际细胞系认证委员会 (International Cell Line Authentication Committee, ICLAC)、美国典型培养物保藏中心 (American Type Culture Collection, ATCC)等权威机构将STR分型视为细胞株鉴定的金标准方法。4. 什么是STR分型技术?答:STR(Short Tandem Repeat)...
例如ICLAC、欧洲认证细胞培养物收藏中心 (EuropeanCollection of Authenticated Cell Cultures, ECACC) 这些机构都建立了关于细胞株鉴定的建议和指南。ATCC的标准发展组织 (Standards Development Organization, SDO) 颁布了ASN-0002标准《Authentication of Human Cell Lines: Standardization of STR Profiling》。 4.采用STR...
关键字:MT-2 Cell Lines:人淋巴瘤; 公司简介 上海冠导生物工程有限公司,先后从ATCC、DSMZ、ECACC、RIKEN、PromoCell、ScienCell、JCRB等国内外细胞库引进细胞2000余株。以此为契机,公司组建了冠导细胞库,我司细胞均由资深细胞培养工程师进行培养。我司可以提供的细胞有:①细胞系②原代细胞③稳转株④耐药株⑤标记细...
3. Korch CT et al. Human Cell Line Authentication: Standardization of Short Tandem Repeat (STR) Profiling, ASN-0002-2022. ATCC Standards Development Organization ASN-0002-2022. Manassas, VA, USA.4. Almeida J. L & Korch C.T. Authentication of Human and Mouse Cell Lines by Short Tandem ...
关键字:OCI-Ly1 Cell Lines:人; 公司简介 上海冠导生物工程有限公司,先后从ATCC、DSMZ、ECACC、RIKEN、PromoCell、ScienCell、JCRB等国内外细胞库引进细胞2000余株。以此为契机,公司组建了冠导细胞库,我司细胞均由资深细胞培养工程师进行培养。我司可以提供的细胞有:①细胞系②原代细胞③稳转株④耐药株⑤标记细胞⑥...
Purchase A549 cell line (ATCC) A549 Cell (Wikipedia) Proven transduction protocols for the most popular cancer cell lines and varieties are introduced. These arrangements assist you in receiving the outcome you hope to get the first time with few expectations. Search for your specific cell line ...
This includes the lack of malignant prostate cell lines that are needed for this highly malignant cancer. Prostate cancer cell lines that are likely candidates for xenograft studies end up losing expression of key proteins (i.e. hPAP, PSA). Multiple groups have reported on the genetic variation...
关键字:C8166 Cell Lines:人T淋; 公司简介 上海冠导生物工程有限公司,先后从ATCC、DSMZ、ECACC、RIKEN、PromoCell、ScienCell、JCRB等国内外细胞库引进细胞2000余株。以此为契机,公司组建了冠导细胞库,我司细胞均由资深细胞培养工程师进行培养。我司可以提供的细胞有:①细胞系②原代细胞③稳转株④耐药株⑤标记细胞...