Since upregulation of Diap1-GFP in the F-Cell occurs by 38–40 hAPF, well after the division of the bristle lineage cells (Extended Data Fig. 1b,c), we hypothesized that F-Cells might be specified post-mitotically. To test this hypothesis, we used a high-power femtosecond laser ...
Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that ...
a A schematic diagram displaying the artificial division of the three compartments (cortex, medulla, and pelvis regions) of the kidney based on histomorphology. t-SNE shows the cell types identified with all the single cells, and colors indicate the 15 spatial clustering assignments; b Multimodal...
What role does IL-17A signaling play in secretory cell lineage commitment within the intestinal epithelial compartment? What evidence suggests that IL-17A acts on Lgr5 + Intestinal Stem Cells (ISCs) rather than Atonal Homolog 1 (ATOH1) + secretory progenitor cells? How does IL-17RA signaling ...
The detailed protocol described in this research encompasses the entire process in a detailed manner, from the expansion of PB-MNCs and generation of iPSCs by transfection of episomal vectors, maintenance of iPSCs, and differentiation into erythroid lineage cells. Furthermore, this process makes use...
Venn diagram showing the overlap between the most abundant TCR sequences detected by each method. The threshold was defined by the method which detected fewest TCR species (UMI-corrected 5’RACE-based PCR). The diagrams show the number and relative frequency of TCR sequences detected by only on...
From 22 cell subsets of CIBERSORT output using LM22 matrix, “B cells naïve,”“B cells memory” and “plasma cells” were combined as “B cell lineage”; “T cells CD4 memory resting” and “T cells CD4 memory activated” were combined as “CD4 memory T cells”; “NK cells resting...
Three-layered models connecting molecular aberrations and gene expressions. Left: layer 1 models connecting mutations, CNVs, DNA methylations with mRNA and protein expressions of the same genes (g1in the diagram). Solid black lines indicate transcription and translation. Solid green lines indicate lay...
Comparison of DE genes identified by Granatum or ASAP pipeline.aMA plot.Blue colorlabels DE genes, andgray dotsare non-DE genes.bVenn diagram showing the number of DE genes identified by both methods, as well as those uniquely identified by either pipeline.cBar chart comparing the number of ...
Figure S1. Venn diagram showing the overlap between the most abundant TCR sequences detected by each method. The threshold was defined by the method which detected fewest TCR species (UMI-corrected 5’RACE-based PCR). The diagrams show the number and relative frequency of TCR sequences ...