We observed that the majority of CellTag reads mapped to the expected species of origin, 87.2% for the mouse library and 91.4% for the human library. (i) Bar plot showing cross-talk levels (Methods) across the human and mouse cells profiled. (j) Line plots showing relative abundance of...
As the field of eukaryogenesis has been confused by a plethora of contradictory ideas, some not compatible with established evidence, before presenting the novel explanations I summarise two areas to put them in context: (1) the phylogenetic origin of the eukaryotic components, and (2) the orig...
these data show that overexpression of a single chRCC master transcription-factor candidate,FOXI1, in the ccRCC cell line 786-O, with or without knockdown ofEPAS1, led to marked expression changes driving the cell line to be more like a chRCC cell line without any modification to the set ...
In the intestine, the identity of the stem cell is still under debate. Two different models were proposed so far: the +4 model and the stem cell zone model. In the +4 model, cell-tracking experiments predicted a common cell of origin at position 4–5 in the intestinal crypt, just abov...
Plant cell walls are highly dynamic and chemically complex structures surrounding all plant cells. They provide structural support, protection from both ab
Novel triple-positive markers identified in human non-small cell lung cancer cell line with chemotherapy-resistant and putative cancer stem cell characteristics. Oncol Rep. 2018;40:669–81. PubMed PubMed Central Google Scholar Zhang Y, Xu W, Guo H, et al. NOTCH1 signaling regulates self-...
Although the origin of the eukaryotic cell has long been recognized as the single most profound change in cellular organization during the evolution of life on earth, this transition remains poorly understood. Models have always assumed that the nucleus and endomembrane system evolved within the cytopl...
We found that introduction of ESCs decreased the number of inner, apolar blastomeres at the 8- to 16-cell stage transition and reduced the number of ICM cells of host embryo-origin during formation of the blastocyst. Moreover, we showed that the proportion of inner blastomeres and their fate...
Molecular profiling of single cells has advanced our knowledge of the molecular basis of development. However, current approaches mostly rely on dissociating cells from tissues, thereby losing the crucial spatial context of regulatory processes. Here, we
Finally, Huatuo combines the genetic associations with gene expression levels, including both standard eQTLs and cell cluster-ieQTLs, to infer genes that can be perturbed by the putative functional genetic variation. Full size image In this study, we applied Huatuo to the single-cell transcriptome ...