6.1. Cell line cultures development The first cell line—the “L” cell line—was established by Earle in 1948. This cell line was derived from subcutaneous mouse tissue [70, 71] and displayed quite different morphology from the origin of tissue [70]. In 50s and 60s, another diploid cell...
We observed that the majority of CellTag reads mapped to the expected species of origin, 87.2% for the mouse library and 91.4% for the human library. (i) Bar plot showing cross-talk levels (Methods) across the human and mouse cells profiled. (j) Line plots showing relative abundance of...
We then removed segmented regions most likely to correspond to empty space rather than cell-containing regions by testing whether a putative cell’s square root-transformed segmented area was larger than expected (Z test; FDR threshold of 0.01). Of the remaining segmented regions, we considered ...
Novel triple-positive markers identified in human non-small cell lung cancer cell line with chemotherapy-resistant and putative cancer stem cell characteristics. Oncol Rep. 2018;40:669–81. PubMed PubMed Central Google Scholar Zhang Y, Xu W, Guo H, et al. NOTCH1 signaling regulates self-...
In the intestine, the identity of the stem cell is still under debate. Two different models were proposed so far: the +4 model and the stem cell zone model. In the +4 model, cell-tracking experiments predicted a common cell of origin at position 4–5 in the intestinal crypt, just abov...
The prevalence of kidney diseases is emerging as a public health problem. Stem cells (SCs), currently considered as a promising tool for therapeutic application, have aroused considerable interest and expectations. With self-renewal capabilities and grea
The human diploid dermal fibroblast cell line was established at Manipal School of Life Sciences, MAHE, and Manipal. SiHa and CaSki cell lines were maintained in DMEM and RPMI media supplemented with 10% fetal bovine serum (FBS). Tumor cell lines were authenticated for its origin through STR ...
We found that introduction of ESCs decreased the number of inner, apolar blastomeres at the 8- to 16-cell stage transition and reduced the number of ICM cells of host embryo-origin during formation of the blastocyst. Moreover, we showed that the proportion of inner blastomeres and their fate...
MitoCIAO identifies putative interactors of OPA1 MitoCIAO识别OPA1的潜在互作蛋白 Para_01 在验证了MitoCIAO和MARIGOLD与已知的MICOS复合体后,我们接下来希望通过探索OPA1的动态复合物来利用其作为发现工具的潜力,OPA1是另一个关键的嵴调节因子。 因此,我们在MitoCIAO中查询了OPA1,并获得了前50个候选OPA1互作蛋白...
(Fig.4c). The cluster of neural stem cells is well recognized as the origin cell-type with outward velocity arrows and a low pseudotime index, while the GABA interneurons are confirmed as a terminal cell-type with incoming velocity arrows and a high pseudotime index. In comparison, the ...