Development and validation of a protocol for cell line identification by MALDI-TOF MS. BMC Proc. 2011;5 Suppl 8:45. 25. Hanrieder J, Wicher G, Bergquist J, Andersson M, Fex-Svenningsen A. MALDI mass spectrometry based molecular phenotyping of CNS glial cells for prediction in mammalian ...
When you are ready to transfer your protocol to large-scale manufacturing, establishment of a stable cell line is the right choice. Primary cells differ from cell lines because they have a limited life span and therefore the duration of primary cell culture depends heavily on the cell type. ...
Single-stream, animal origin free medium for the entire workflow, from cell line to process development Complete protocol provided Proven for IgG production Learn more › Order now Catalog #NameSizePrice (CNY)Qty A46847Freedom™ ExpiCHO-S™ KitEach ...
Single-stream, animal origin free medium for the entire workflow, from cell line to process development Complete protocol provided Proven for IgG production Learn more › Order now Catalog #NameSizePrice (CNY)Qty A46847Freedom™ ExpiCHO-S™ KitEach ...
The A549 cell line is a widely used human lung adenocarcinoma cell line that was derived from a primary lung tumor. A549 cells are highly proliferative and have been used in a wide variety of research applications.
Remove medium. Wash cells with calcium- and magnesium-free balanced salt solution (phosphate buffered saline, Hanks’ balanced salt solution, etc. Add detaching agent (e.g., trypsin). Incubate at 37°C until cells are fully detached from the dish (2–20 min depending on the cell line). ...
For high through-put single-cell studies, a 10X Genomics Chromium machine was used for 3000–5000 single-cell capture and cDNA preparation according to the Single Cell 3′ Protocol recommended by the manufacturer. The cells are first washed and prepared in an ideal concentration. The machine part...
Brain metastasis (BrM) poses a significant challenge to the prognosis and quality of life for patients with non-small cell lung cancer (NSCLC). Gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter in the central nervous system (CNS), has been i
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We also performed cell cycle analysis following a protocol in Seurat 3 using 70 cell cycle markers79and found that, for both wild-type andAtoh7-null cells, the three naive progenitor cell clusters C0–C2 roughly co-segregated with their positions in the cell cycle, C0 in G1 and early S,...