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The human neuroblastoma cell line, SH-SY5Y, is a commonly used cell line in studies related to neurotoxicity, oxidative stress, and neurodegenerative diseases. Although this cell line is often used as a cellular model for Parkinson’s disease, the relevance of this cellular model in the context ...
Transfection in HN5 cells: For each transfection, 1.6 μg of plasmid DNA has been incubated for 20 min with 4 µl of Lipofectamine 2000 transfection reagent (Invitrogen) in 0.2 ml of OptiMEM media (Gibco) and added to the cells cultured in a six-well plate. As all plasmids...
1e6 cells were pipetted out from each cell line and stained with Vybrant DyeCycle orange stain (Invitrogen). Reporting summary Further information on research design is available in the Nature Research Reporting Summary linked to this article....
An Invitrogen Attune NxT flow cytometer and Invitrogen Attune NxT software were used for data collection. Cell sorting was performed with a Sony MA900 flow cytometer. Data were analyzed with FlowJo software. For detailed antibody information, please see Supplementary Table 8. Digital western blotting...
Total RNA was isolated using Trizol (Invitrogen) from three replicate samples of BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2-43 for 48 h. Affymetrix microarray was performed using the Human Gene 1.0 ST microarray chip (Agilent Technologies, Santa Clara, CA, ...
Thymic lobes were isolated from E14.5 embryos and cultured for 4 days on nucleopore filters (Whatmann, NJ) placed in RPMI1640 (Invitrogen), supplemented with 10% fetal bovine serum (Invitrogen), 2 mM L-glutamine (Invitrogen), 50 µM 2-mercaptoethanol (Sigma-Aldrich) and 1.35 mM 2...
The ciliated epithelial cells line the extensive surface of the respiratory tract between the nose and the alveoli in the lung and are heavily exposed to viral and bacterial pathogens.33 Ciliated epithelial cells in the nose and conducting airways, the bronchi and bronchioles, have been found to ...
CNE cell line was purchased from Kunming Cell Bank (China) and cultured in Dulbecco's modified Eagle's medium (Gibco/Invitrogen, 12800-017) supplemented with 10% fetal bovine serum (PAA, A15-101) at 37°C in a humidified 5% CO2 incubator. Cells were transfected with siRNA or miRNAs duplex...
Total RNA from 2 Gy irradiated breast cancer cells was extracted using TRIzol (Invitrogen) 24 hours after irradiation, then quantified and diluted to reach the concentration of 50 nanogram per each µliter. The concentration of RNA was measured by NanoDrop 3300 spectrometer (Thermo Scientific...